Effect of Royal Jelly on the Fertilizing Ability of Buffalo Spermatozoa In Vitro

Authors

  • Saber Mohamed Abd-Allah Faculty of Veterinary Medicine, Beni-Suef University, 62511 Beni-Suef, Egypt

DOI:

https://doi.org/10.6000/1927-520X.2012.01.01.01

Keywords:

Buffalo, Capacitation, Spermatozoa, IVF, Royal Jelly

Abstract

The aim of the present study was to assess the effect of addition of Royal jelly in presence of heparin on buffalo (Bubalus Bubalis) sperm motility, acrosome reaction and in vitro fertilization (IVF) of buffalo oocytes. Frozen buffalo spermatozoa from five bulls were thawed and motile fraction was obtained by swim up technique. The spermatozoa were washed, treated with100 µg/ml heparin, and then exposed to 0.4% Royal Jelly (RJ) for 3 h. Sperm motility, acrosomal integrity and fertilization rate of matured oocytes were assessed at 1, 2 and 3 h. The percentages of sperm motility, intact acrosome and fertilization rate of matured oocytes were higher (P<0.05) in 0.4% RJ compared to that in the control. After 2 h of incubation the percentage of motility, intact acrosome of spermatozoa and fertilization rate of matured oocytes, respectively, were 93.6 %, 77.6% and 72.6% in 0.4% RJ. These results suggest that treating buffalo sperm with 0.4% RJ in combination with heparin is effective not only to induce sperm acrosome reaction but also is effective for in vitro fertilizing capacity of the cryopreserved buffalo spermatozoa.

References

Johnson LA, Weitze KF, Fiser P, et al. Storage of boar. Anim Reprod Sci 2000; 62: 143-72. http://dx.doi.org/10.1016/S0378-4320(00)00157-3

Holt WV. Fundemental aspect of sperm. Theriogenology 2000; 43: 47-5. http://dx.doi.org/10.1016/S0093-691X(99)00239-3

Abd-Allah SM. Ameliorating effect of royal jelly. Annales UMCS, Med Vet 2010; 65: 1-8.

Abd-Allah SM. Laboratory production of buffalo embryos,

st ed. Lambert VDM-Germany 2011; pp. 217.

Hove SR, Dimick PS, Benton AW. Composition of fresh. J Apic Res 1985; 24: 52-1.

Kodai T, Umebayashi K, Nakatani T, et al. Compositions of royal jelly II. Chem Pharm Bull 2007; 55: 1528-31. http://dx.doi.org/10.1248/cpb.55.1528

Intani A, Kasai M, Niwa K, Song H. Fertilization in vitro. J Reprod Fertil 1984; 70: 487-92.

Parrish JJ, Susko-Pamsh JL, Winter MA, et al. Capacitation of bovine sperm. Biol Reprod 1988; 38: 1171-80. http://dx.doi.org/10.1095/biolreprod38.5.1171

Byrd W. In vitro capacitation. J Exp Zool 1981; 215: 35-6. http://dx.doi.org/10.1002/jez.1402150105

Vijayaraghavan S, Hoskins D. Regulation of bovine sperm. Biol Reprod 1986; 34: 468-77. http://dx.doi.org/10.1095/biolreprod34.3.468

Fraser LR, Duncan AE. Adenosine analogues. J Reprod Fertil 1993; 187-194. http://dx.doi.org/10.1530/jrf.0.0980187

Green CM, Cockle SM, Watson PF, et al. A possible mechanism. Mol Reprod Dev1996; 45: 244-52. http://dx.doi.org/10.1002/(SICI)1098-2795(199610)45:2<244:: AID-MRD19>3.3.CO;2-7

Zemjanis R. Collection and evaluation of semen. [In:] Diagnostic and therapeutic techniques in animal reproduction. William and Wilkins Company, Baltimore, USA 1977; pp. 242.

Sidhu KS, Dhindsa JS. Guraya SS. A simple staining procedure. Biotech Histochem 1992; 67: 35-9. http://dx.doi.org/10.3109/10520299209110003

Abd-Allah SM. In vitro Embryo. Vet Ital 2009; 45: 425-9.

Snedecor GW, Cochran WF. Statistical methods. 6th ed. Ames. Iowa State University Press 1967; pp. 593.

Downloads

Published

2012-03-01

How to Cite

Abd-Allah, S. M. (2012). Effect of Royal Jelly on the Fertilizing Ability of Buffalo Spermatozoa In Vitro. Journal of Buffalo Science, 1(1), 1–4. https://doi.org/10.6000/1927-520X.2012.01.01.01

Issue

Section

Articles