Evaluation of the Effects of Bio-Stimulation and Gonadotropin-Releasing Hormone on First-Service Conception Rate in Buffalo Cows

Ashit Kumar  Paul; Md.  Fakruzzaman; Md. Ashadul  Alam; Gautam Kumar  Deb; MAM Yahia  Khandoker

doi:10.6000/1927-520X.2026.15.06

Authors

Ashit Kumar Paul Department of Medicine, Surgery and Obstetrics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal Campus, Barishal, Bangladesh
Md. Fakruzzaman Department of Genetics and Animal Breeding, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal Campus, Barishal, Bangladesh
Md. Ashadul Alam Buffalo Research and Development Project, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh
Gautam Kumar Deb Buffalo Research and Development Project, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh
MAM Yahia Khandoker Department of Animal Breeding and Genetics, Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh, Bangladesh

DOI:

https://doi.org/10.6000/1927-520X.2026.15.06

Keywords:

Artificial insemination, GnRH, mPLD, Pregnancy, River-type buffalo

Abstract

This study investigated the efficacy of combining bio-stimulation and hormonal interventions to enhance the first AI conception rate (CR-FAI) in buffaloes. Under field conditions, 224 adult female buffaloes were randomly assigned (lottery method) to one of two treatment protocols. In group A, AI was conducted using a modified penis-like device (mPLD) and the application of clitoral massage (CM) for 30 seconds (AI + mPLD + CM). In group B, AI included mPLD and CM supplemented with an intramuscular injection of 500 µg of gonadotrophin-releasing hormone (GnRH) immediately post-insemination (AI + mPLD + CM + GnRH). Confounding variables, including age, parity, body condition score (BCS), and breed of the buffaloes, were recorded at the time of AI. The results demonstrated that the CR-FAI in group B (77.4%) was significantly higher than in group A (63.6%; p < 0.05). While age categories exhibited significant variation in CR-FAI (p < 0.05; χ2 = 8.80), no significant variation was found for parity, BCS, or breed. Logistic regression revealed that buffaloes at parity 1 and 2 possessed 3.6-times and 2.7-times higher odds of conception, respectively, [Wald=1.02, OR =3.61, 95% CI (0.30-43.99) and Wald =1.81, OR =2.65, 95% CI (0.64-11.0)], relative to other parity groups. Furthermore, age was highly significant and positively correlated with parity and BCS (p <0.01). In conclusion, AI integrating bio-stimulation with concurrent GnRH administration substantially enhances the conception rates in buffaloes.

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