Biochemical Profile of Milk of Buffalo (Bubalus bubalis), Cow (Bos taurus) and Goat (Capra hircus): Potential Candidates for Supporting the Growth of Leishmania donovani Promastigotes in Culture Medium as Alternative to Fetal Bovine Serum (FBS)
DOI:
https://doi.org/10.6000/1927-520X.2012.01.02.07Keywords:
Biochemical profile, milk of buffalo, cow, goat, Leishmania donovani, culture medium, alternative, fetal bovine serumAbstract
The milk of cow (Bos taurus), buffalo (Bubalus bubalis), and goat (Capra hircus) were investigated for thebiochemical profile such as glucose, total protein, triglycerides, cholesterol and calcium. Although used as potentialcandidate to replace fetal bovine serum in the culture medium for the cultivation of parasitic protozoan such asLeishmania donovani promastigotes, the biochemical profile of milk will provide information about which factor supportsand essential for the growth of parasite. We found triglycerides were predominant in goat followed by cow and buffalomilk. The level of cholesterol was high in buffalo milk followed by goat and FBS. The level of calcium was high in buffalomilk followed by goat and cow milk.References
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http://dx.doi.org/10.1128/CMR.15.3.327-328.2002
[2] Newman C. Serum-free cell culture—. Biomed Scientist, September 2003; 941-2.
[3] Ali SA, Iqbal J, Ahmad B, Masoom M. A semi synthetic fetal calf serum-. Am J Trop Med Hyg 1998; 59: 163-5.
[4] Armstrong TC, Patterson JL. Cultivation of Leishmania braziliensis. J Parasitol 1994; 80: 1030-2.
http://dx.doi.org/10.2307/3283454
[5] Chaudhuri G, Ghoshal K, Sen S, Pal S, Banerjee AB. Nutrition of Leishmania donovani donovani:. Indian J Med Res 1986; 84: 461-8.
[6] Merlen T, Sereno D, Brajon N, Rostand F, Lemesre JL. Leishmania spp.: completely. Am J Trop Med Hyg 1999; 60: 41-50.
[7] Pal JK, Purandare MJ. Dose-dependent differential effect of hemin. J Biosci 2001; 26: 225-31.
http://dx.doi.org/10.1007/BF02703646
[8] Schuster FL, Sullivan JJ. Cultivation of clinically. Clin Microbiol Rev 2002; 15: 374-89.
http://dx.doi.org/10.1128/CMR.15.3.374-389.2002
[9] Shamsuzzaman, SM, Furuya M, Korenaga M, Imamura K, Hashigughi Y. Use of urine samples. Ann Trop Med Parasitol 1999; 93: 613-20.
[10] Muniaraj M, Lal CS, Kumar S, Sinha PK, Das P. Milk of Cow (Bos taurus), Buffalo (Bubalus bubalis), and Goat (Capra hircus): a Better Alternative J Clin Microbiol 2007; 45(4): 1353-56.
http://dx.doi.org/10.1128/CMR.15.3.327-328.2002
[2] Newman C. Serum-free cell culture—. Biomed Scientist, September 2003; 941-2.
[3] Ali SA, Iqbal J, Ahmad B, Masoom M. A semi synthetic fetal calf serum-. Am J Trop Med Hyg 1998; 59: 163-5.
[4] Armstrong TC, Patterson JL. Cultivation of Leishmania braziliensis. J Parasitol 1994; 80: 1030-2.
http://dx.doi.org/10.2307/3283454
[5] Chaudhuri G, Ghoshal K, Sen S, Pal S, Banerjee AB. Nutrition of Leishmania donovani donovani:. Indian J Med Res 1986; 84: 461-8.
[6] Merlen T, Sereno D, Brajon N, Rostand F, Lemesre JL. Leishmania spp.: completely. Am J Trop Med Hyg 1999; 60: 41-50.
[7] Pal JK, Purandare MJ. Dose-dependent differential effect of hemin. J Biosci 2001; 26: 225-31.
http://dx.doi.org/10.1007/BF02703646
[8] Schuster FL, Sullivan JJ. Cultivation of clinically. Clin Microbiol Rev 2002; 15: 374-89.
http://dx.doi.org/10.1128/CMR.15.3.374-389.2002
[9] Shamsuzzaman, SM, Furuya M, Korenaga M, Imamura K, Hashigughi Y. Use of urine samples. Ann Trop Med Parasitol 1999; 93: 613-20.
[10] Muniaraj M, Lal CS, Kumar S, Sinha PK, Das P. Milk of Cow (Bos taurus), Buffalo (Bubalus bubalis), and Goat (Capra hircus): a Better Alternative J Clin Microbiol 2007; 45(4): 1353-56.
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Published
2012-08-29
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Muniaraj, M., Kumar, S., Lal, C. S., & Sinha, P. K. (2012). Biochemical Profile of Milk of Buffalo (Bubalus bubalis), Cow (Bos taurus) and Goat (Capra hircus): Potential Candidates for Supporting the Growth of Leishmania donovani Promastigotes in Culture Medium as Alternative to Fetal Bovine Serum (FBS). Journal of Buffalo Science, 1(2). https://doi.org/10.6000/1927-520X.2012.01.02.07
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