Pregnancy Outcomes Following GnRH- or Prostaglandin-Based Timed Artificial Insemination Protocols in Water Buffaloes (Bubalus bubalis)

Authors

  • E.C. Atabay Philippine Carabao Center at CLSU, Science City of Muñoz, Nueva Ecija, 3120, Philippines and Reproduction and Physiology Section, Philippine Carabao Center, National Headquarters, Science City of Muñoz, Nueva Ecija, 3120 Philippines
  • E.P. Atabay Reproduction and Physiology Section, Philippine Carabao Center, National Headquarters, Science City of Muñoz, Nueva Ecija, 3120 Philippines
  • C.F. Dela Cruz Reproduction and Physiology Section, Philippine Carabao Center, National Headquarters, Science City of Muñoz, Nueva Ecija, 3120 Philippines https://orcid.org/0009-0006-8222-3444
  • A.V. Ferrer College of Agriculture, Department of Animal Science, Bulacan Agricultural State College, San Ildefonso, Bulacan 3010, Philippines

DOI:

https://doi.org/10.6000/1927-520X.2024.13.10

Keywords:

GnRH, hCG, progesterone, prostaglandin, timed artificial insemination, water buffaloes

Abstract

The efficiencies of Timed Artificial Insemination (TAI) protocols in post-partum riverine dairy buffaloes were evaluated in the present research work. In Study 1, GnRH-based ovulation synchronization for Fixed Time Artificial Insemination (FTAI) protocol was evaluated for pregnancy. Buffaloes in Treatment 1 were subjected to the Ovsynch (GnRH-PGF2α-GnRH) protocol. Buffaloes in Treatment 2 were subjected to Controlled Internal Drug Release–Gonadotrophin Releasing Hormone (CIDR-Synch-GnRH) protocol, and buffaloes in Treatment 3 were subjected to CIDR-Synch-human Chorionic Gonadotrophin (CIDR-Synch-hCG) protocol. In Study 2, Prostaglandin-based estrus synchronization protocols were similarly evaluated for pregnancy. Buffaloes in Treatment 1 were treated with Prostaglandin hormone alone; buffaloes in Treatment 2 were subjected to Prostaglandin-GnRH protocol, while buffaloes in Treatment 3 were subjected to Prostaglandin-hCG protocol. Results in Study 1 revealed that supplementation of Ovsynch with CIDR in Treatment 2 and 3 resulted in significantly higher (P<0.05) pregnancy rates compared with Ovsynch alone (T1). Meanwhile, the use of hCG as the final ovulatory hormone in FTAI protocol (T3) yielded a significantly higher (P<0.05) pregnancy rate than GnRH (T2). In Study 2, results showed that prostaglandin protocols enhanced with GnRH (T2) or with hCG (T3) resulted in significantly higher (P<0.05) pregnancy rates (31.88±3.39 and 34.62±1.53), respectively, compared with Prostaglandin alone (T1, 23.91±2.49). However, pregnancy rates in Prostaglandin-based protocols (T2) and (T3) were not significantly different (P<0.05). In sum, the present study demonstrated that supplementation with exogenous progesterone (CIDR) improved the efficiency of Ovsynch FTAI protocol while using hCG as the final ovulatory hormone is found to be the best among FTAI protocols. Meanwhile, a Prostaglandin-based protocol enhanced with ovulatory hormones, either GnRH or hCG, on the day of AI improved pregnancy rates in post-partum water buffaloes.

References

Moore SG, Hasler JF. A 100-Year Review: Reproductive technologies in dairy science. J Dairy Sci 2017; 100:10314-10331. https://doi.org/10.3168/jds.2017-13138 DOI: https://doi.org/10.3168/jds.2017-13138

Nanda AS, Brar PS, Prabhakar S. Enhancing reproductive performance in dairy buffalo: Major constraints and achievements. Reproduction 2003; 61: 27-36.

Das GK, Khan FA. Summer anestrus in buffalo - A review. Reprod. Domest. Anim 2010; 45: 483-494. https://doi.org/10.1111/j.1439-0531.2010.01598.x DOI: https://doi.org/10.1111/j.1439-0531.2010.01598.x

Borghese A, Barile V, Terzano G, Annicchiarico G, Debenedetti A, Malfatti A. In: Anoestrus length in Italian buffalo cows. Note I 1993; 389-392.

Pursley JR, Kosorok MR, Wiltbank, WC. Reproductive management of lactating dairy cows using synchronization of ovulation. J. Dairy Sci. 1997; 80: 301-306. https://doi.org/10.3168/jds.S0022-0302(97)75938-1 DOI: https://doi.org/10.3168/jds.S0022-0302(97)75938-1

Pursley JR, Silcox RW, Wiltbank MC. Effect of time of artificial insemination on pregnancy rates, calving rates, pregnancy loss, and gender ratio after synchronization of ovulation in lactating dairy cows. J. Dairy Sci 1998; 81: 2139-2144. https://doi.org/10.3168/jds.S0022-0302(98)75790-X DOI: https://doi.org/10.3168/jds.S0022-0302(98)75790-X

Dalton JC, Nadir S, Bame JH, Noftsinger M, Nebel RL. Saacke RG. Effect of time of insemination on number of accessory sperm, fertilization rate, and embryo quality in nonlactating dairy cattle. J. Dairy Sci 2001; 84: 2413- 2418. https://doi.org/10.3168/jds.S0022-0302(01)74690-5 DOI: https://doi.org/10.3168/jds.S0022-0302(01)74690-5

Moreira F, Orlandi C, Risco CA, Mattos R, Lopes F, Thatcher WW. Effects of presynchronization and bovine somatotropin on pregnancy rates to a timed artificial insemination protocol in lactating dairy cows. J Dairy Sci 2001; 84: 1646-1659. https://doi.org/10.3168/jds.S0022-0302(01)74600-0 DOI: https://doi.org/10.3168/jds.S0022-0302(01)74600-0

Stevenson JS, Tiffany SM, Lucy MC. Use of estradiol cypionate as a substitute for GnRH in protocols for synchronizing ovulation in dairy cattle. J. Dairy Sci 2004; 87: 3298-3305. https://doi.org/10.3168/jds.S0022-0302(04)73466-9 DOI: https://doi.org/10.3168/jds.S0022-0302(04)73466-9

Lamb GC, Cartmil JAI, Stevenson AS. Effectiveness of select synch gonadotropin-releasing hormone and prostaglandin F2α for synchronizing estrus in replacement beef heifers. Professional Animal Scientist 2004; 20: 27. https://doi.org/10.15232/S1080-7446(15)31268-7 DOI: https://doi.org/10.15232/S1080-7446(15)31268-7

Amaya-Montoya C, Matsui M, Kawashima C, Hayashi KG, Matsuda G, Kaneko F, Kida K, Miyake Y. Induction of ovulation with GnRH and PGF2α at two different stages during the early post-partum period in dairy cows’ ovarian response and changes in hormone concentration. J. Reprod Dev 2007; 53-867. https://doi.org/10.1262/jrd.18163 DOI: https://doi.org/10.1262/jrd.18163

Alapati A, Kapa SR, Jeepalyam S, Rangappa SM, KR. Development of the body condition score system in Murrah buffaloes, validation through ultrasonic assessment of the body fat reserve. J Vet Sci 2010; 11: 1-8. https://doi.org/10.4142/jvs.2010.11.1.1 DOI: https://doi.org/10.4142/jvs.2010.11.1.1

Mehrajuddin N, Patel DM, Derashri HJ. Early pregnancy diagnosis by transrectal ultrasonography in Mehsana buffaloes (Bubalus bubalis). Buffalo Bulletin 2013; 32 (2): 120-125.

Fricke PM, Ricci A, Giordano JO, Carvalho PD. Methods for implementation of pregnancy diagnosis in dairy cows. Veterinary Clinics of North America: Food Animal Practice 2016; 32: 165–180. https://doi.org/10.1016/j.cvfa.2015.09.006 DOI: https://doi.org/10.1016/j.cvfa.2015.09.006

Pursley JR, Mee MO, Wiltbank MC. Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology 1995; 44: 915-923. https://doi.org/10.1016/0093-691X(95)00279-H DOI: https://doi.org/10.1016/0093-691X(95)00279-H

Baruselli PS, Reis EL, Marques MO, Nasser LF, Bó GA.The use of hormonal treatments to improve reproductive performance of anestrous beef cattle in tropical climates Animal Reproduction Science 2004; 479–486. https://doi.org/10.1016/j.anireprosci.2004.04.025

De Rensis F, Ronci G, Guarneri P, Nguyen BX, Presicce GA, Huszenicza G, Scaramuzzi RJ. Conception rate after fixed time insemination following ovsynch protocol with and without progesterone supplementation in cyclic and non-cyclic Mediterranean Italian buffaloes (Bubalus bubalis), Theriogenology 2005; 63: 1824-1831. https://doi.org/10.1016/j.theriogenology.2004.07.024 DOI: https://doi.org/10.1016/j.theriogenology.2004.07.024

Campanile G, Vecchio D, Neglia G, Di Palo R, Prandi A, Occhio MJ. Progesterone and pregnancy status in buffaloes treated with a GnRH agonist. Livest. Sci 2008; 115(2-3). https://doi.org/10.1016/j.livsci.2007.08.001 DOI: https://doi.org/10.1016/j.livsci.2007.08.001

Paul V, Prakash BS. Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in Murrah buffaloes (Bubalus bubalis) Theriogenology 2005; 64(5):1049-1060. https://doi.org/10.1016/j.theriogenology.2005.02.004 DOI: https://doi.org/10.1016/j.theriogenology.2005.02.004

Purohit GN, Thanvi P, Pushp M, Gaur M, Saraswat CS, Arora AS, Pannu SP, Goche T. Estrus synchronization in buffaloes: Prospects, approaches, and limitations. The Pharma Innovation Journal 2019; 8(2): 54-62.

De Rensis F, Lopez-Gatius F. Protocols for synchronization estrus and ovulation in buffalo (Bubalus bubalis): A review. Theriogenology2007; 67: 209-216. https://doi.org/10.1016/j.theriogenology.2006.09.039 DOI: https://doi.org/10.1016/j.theriogenology.2006.09.039

Fortune JE. Follicular dynamics during the bovine estrous cycle: A limiting factor in the improvement of fertility. Animal Reproduction Science 1993; 33(1-4): 111-125. https://doi.org/10.1016/0378-4320(93)90110-D DOI: https://doi.org/10.1016/0378-4320(93)90110-D

Savio JD, Thatcher WW, Morris GR, Entwistle K, Drost M, Mattiacci MR. Effects of induction of low plasma progesterone concentrations with a progesterone-releasing intravaginal device on follicular turnover and fertility in cattle. J. Reprod. Fert 1993; 98(1): 77-84. https://doi.org/10.1530/jrf.0.0980077 DOI: https://doi.org/10.1530/jrf.0.0980077

Baruselli PS, Reis EL, Marquez MO, Nasser LF, Bo GA. The use of hormonal treatment to improve the reproductive performance of beef cattle in tropical climates. Ani Repro Sci. 2004; 82-83: 79 486. https://doi.org/10.1016/j.anireprosci.2004.04.025 DOI: https://doi.org/10.1016/j.anireprosci.2004.04.025

Atabay EP, Atabay EC, Maylem ERS, Tilwani RC, Flores EB, Sarabia AS. Improved pregnancy in Water Buffaloes through synchronization of ovulation and fixed-time artificial insemination technique. Philipp J Vet Med 2019; 56(2): 1-9.

De Rensis F, Marconi P, Capelli T, Gatti F, Facciolongo F, Franzini S, Scaramuzzi RJ. Fertility in post-partum dairy cows in winter or summer following estrus synchronization and fixed time AI after the induction of an LH surge with gonadotropin-releasing hormone (GnRH) or human chorionic gonadotropin (hCG). Theriogenology 2002; 58: 1675-1687. https://doi.org/10.1016/S0093-691X(02)01075-0 DOI: https://doi.org/10.1016/S0093-691X(02)01075-0

Diaz T, Schmitt EJP, dela Sota RL, Thatcher MJ, Thatcher WW. Human Chorionic Gonadotropin-Induced Alterations in Ovarian Follicular Dynamics During the Estrous Cycle of Heifers1. J Anim Sci 1998; 76: 1929–1936. https://doi.org/10.2527/1998.7671929x DOI: https://doi.org/10.2527/1998.7671929x

Breuel KF, Spitzer JC, Henricks DM. Systemic progesterone concentration following human chorionic gonadotropin administration at various times during the estrous cycle in beef heifers. J Anim Sci 1989; 67: 1564−1. https://doi.org/10.2527/jas1989.6761564x DOI: https://doi.org/10.2527/jas1989.6761564x

Geary TW, Salverson RR, Whittier JC. Synchronization of ovulation using GnRH or hCG with the CO-Synch protocol in suckled beef cows. J Anim Sci 2001; 79: 2536-2541. https://doi.org/10.2527/2001.79102536x DOI: https://doi.org/10.2527/2001.79102536x

Baruselli PS. Control of buffalo dynamics for artificial insemination, superovulation, and in-vitro embryo production. Proceedings of the 10th World Buffalo Congress and 7th Asian Buffalo Congress, May 6-8, 2013. Phuket, Thailand.

Atabay EC, Atabay EP, Maylem ERS, Encarnacion EC, Salazar RL. Enhancing prostaglandin-based estrus synchronization protocol for artificial insemination in water buffaloes. Buffalo Bulletin.2020; 39(1): 53-60.

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Published

2024-08-02

How to Cite

Atabay, E. ., Atabay, E. ., Cruz, C. D. ., & Ferrer, A. . (2024). Pregnancy Outcomes Following GnRH- or Prostaglandin-Based Timed Artificial Insemination Protocols in Water Buffaloes (Bubalus bubalis). Journal of Buffalo Science, 13, 90–97. https://doi.org/10.6000/1927-520X.2024.13.10

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