Characterization of Cellular Immune System at Different Ages in Water Buffalo (Bubalus bubalis)

Maria Carmela  Scatà; Giovanna  De Matteis; Francesco  Grandoni; Gabriele  Di Vuolo; Giovanna  Cappelli; Domenico  Vecchio

doi:10.6000/1927-520X.2024.13.15

Authors

Maria Carmela Scatà CREA- Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Research Centre for Animal Production and Aquaculture, Monterotondo, Italy
Giovanna De Matteis CREA- Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Research Centre for Animal Production and Aquaculture, Monterotondo, Italy
Francesco Grandoni CREA- Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Research Centre for Animal Production and Aquaculture, Monterotondo, Italy
Gabriele Di Vuolo Istituto Zooprofilattico Sperimentale del Mezzogiorno, CReNBuf Portici, Italy
Giovanna Cappelli Istituto Zooprofilattico Sperimentale del Mezzogiorno, CReNBuf Portici, Italy
Domenico Vecchio Istituto Zooprofilattico Sperimentale del Mezzogiorno, CReNBuf Portici, Italy

DOI:

https://doi.org/10.6000/1927-520X.2024.13.15

Keywords:

Water buffalo, age, immune system, flow cytometry

Abstract

In recent years, there has been an increased interest in understanding the immune system of the water buffalo due to the increased economic impact of this species. The study aimed to perform an in-depth evaluation of lymphoid and myeloid cells in water buffalo of different ages. We assess three multicolor panels of antibodies to evaluate by flow cytometry the percentage of the CD3⁺ CD4⁺, CD8⁺, and γδ T lymphocytes; CD79⁺ and CD21⁺B lymphocytes; monocytes and cM, intM, and ncMsubsets; NK cells, granulocytes, and peripheral blood mononuclear cell (PBMC). Seventy-eight animals from three different farms were divided into three groups by age (26 in each group): 80-100 days old calves, 16-18 months old heifers, and 4-6 years old cows. Significant differences by Kruskal-Wallis test were found between age groups in the percentage of CD4⁺, CD8⁺, γδ T lymphocytes, NK cells (P=0.0001), total monocytes (P=0.0008), granulocytes (P=0.0358) and PBMC (P=0.0056). Between the farms, the adult animals showed differences in the percentage ofCD3⁺ (P=0.0152), CD4⁺ (P=0.0047), CD8⁺ (P=0.0019), CD4:CD8 ratio (P=0.0033) and γδ (P=0.0013) T lymphocytes; CD21⁺ B lymphocytes (P=0.0007);total monocytes (P=0.0100), cM and ncM subsets (P=0.0320;P=0.0252), granulocytes (P=0.0030) and PBMC (P=0.0120). The calves showed significant differences in CD79⁺ and CD21⁺ B lymphocytes (P=0.0141; P=0.0049), total monocytes (P=0.0010), cM, intM and ncM subsets (P=0.0335; P=0.0499; P=0.0065). The heifers group in CD21⁺ B subset (P=0.0439).In summary, this study provides the composition of lymphoid and myeloid cells in this species for the first time, highlighting large differences between age groups and between different herds.

References

Borghese A, Mazzi M. Buffalo population and strategies in the world. Ed. A. Borghese 2005. In: Buffalo Production and Research, FAO, Rome. Available from: http://www.fao.org/docrep/010/ah847e/ah847e00.htm

Minervino AHH, Zava M, Vecchio D, Borghese A. Bubalus bubalis: A Short Story. Front Vet Sci 2020; 7: 570413. https://doi.org/10.3389/fvets.2020.570413 DOI: https://doi.org/10.3389/fvets.2020.570413

Gabai G, Amadori M, Knight CH, Werling D. The immune system is part of a whole-organism regulatory network. Res Vet Sci 2018; 116: 1-3. https://doi.org/10.1016/j.rvsc.2017.09.018 DOI: https://doi.org/10.1016/j.rvsc.2017.09.018

Chaplin DD. Overview of the immune response. J Allergy Clin Immunol 2010; 125: S3-23. https://doi.org/10.1016/j.jaci.2009.12.980 DOI: https://doi.org/10.1016/j.jaci.2009.12.980

Davis WC, Khalid AM, Hamilton MJ, Ahn JS, Park YH, Cantor GH. The use of cross-reactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis). J Vet Sci 2001; 2: 103-9. https://doi.org/10.4142/jvs.2001.2.2.103 DOI: https://doi.org/10.4142/jvs.2001.2.2.103

Conrad ML, Davis WC, Koop BF. TCR and CD3 antibody cross-reactivity in 44 species. Cytometry A. 2007; 71: 925-33. https://doi.org/10.1002/cyto.a.20435 DOI: https://doi.org/10.1002/cyto.a.20435

Griebel PJ, Entrican G, Rocchi M, Beskorwayne T, Davis WC. Cross-reactivity of mAbs to human CD antigens with sheep leukocytes. Vet Immunol Immunopathol 2007; 119: 115-22. https://doi.org/10.1016/j.vetimm.2007.06.015 DOI: https://doi.org/10.1016/j.vetimm.2007.06.015

Elnaggar MM, Abdellrazeq GS, Sacco RE, Harsla TR, Mucci ML, Fry LM, et al. Comparative analysis of the specificity of monoclonal antibodies developed against the bottlenose dolphin, Tursiops truncatus, TNF-α, IL1-β, IL-6, IL-8, IL-10 with monoclonal antibodies made against ovine IFN-γ bovine IL-17A and IL-1β revealed they recognize epitopes conserved on dolphin and bovine orthologues. Vet Immunol Immunopathol 2022; 250: 110456. https://doi.org/10.1016/j.vetimm.2022.110456 DOI: https://doi.org/10.1016/j.vetimm.2022.110456

Grandoni F, Elnaggar MM, Abdellrazeq GS, Signorelli F, Fry LM, Marchitelli C,et al. Characterization of leukocyte subsets in buffalo (Bubalus bubalis) with cross-reactive monoclonal antibodies specific for bovine MHC class I and class II molecules and leukocyte differentiation molecules. Dev Comp Immunol 2017; 74: 101-109. https://doi.org/10.1016/j.dci.2017.04.013 DOI: https://doi.org/10.1016/j.dci.2017.04.013

Elnaggar MM, Grandoni F, Abdellrazeq GS, Fry LM, El-Naggar K, Hulubei V, et al.The pattern of CD14, CD16, CD163, and CD172a expression on water buffalo (Bubalus bubalis) leukocytes. Vet Immunol Immunopathol. 2019; 211: 1-5. https://doi.org/10.1016/j.vetimm.2019.03.010 DOI: https://doi.org/10.1016/j.vetimm.2019.03.010

Grandoni F, Martucciello A, Petrini S, Steri R, Donniacuo A, Casciari C, et al. Assessment of multicolor flow cytometry panels to study leukocyte subset alterations in water buffalo (Bubalus bubalis) during BVDV acute infection. Front Vet Sci 2020; 7: 574434. https://doi.org/10.3389/fvets.2020.574434 DOI: https://doi.org/10.3389/fvets.2020.574434

De Matteis G, Scatà MC, Zampieri M, Grandoni F, Elnaggar MM, Schiavo L, Cappelli G, Cagiola M, De Carlo E, Davis WC, Martucciello A. Flow cytometric detection of IFN-γ production and Caspase-3 activation in CD4+ T lymphocytes to discriminate between healthy and Mycobacterium bovis naturally infected water buffaloes. Tuberculosis (Edinb). 2023; 139: 102327. https://doi.org/10.1016/j.tube.2023.102327 DOI: https://doi.org/10.1016/j.tube.2023.102327

Grandoni F, Hussen J, Signorelli F, Napolitano F, Scatà MC, De Donato I, Cappelli G, Galiero G, Grassi C, De Carlo E, Petrini S, De Matteis G, Martucciello A. Evaluation of Hematological Profiles and Monocyte Subpopulations in Water Buffalo Calves after Immunization with Two Different IBR Marker Vaccines and Subsequent Infection with Bubaline alphaherpesvirus-1. Vaccines (Basel) 2023; 11: 1405. https://doi.org/10.3390/vaccines11091405 DOI: https://doi.org/10.3390/vaccines11091405

Petrini S, Martucciello A, Grandoni F, De Matteis G, Cappelli G, Giammarioli M, Scoccia E, Grassi C, Righi C, Fusco G, Galiero G, Pela M, De Mia GM, De Carlo E. Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis). Vaccines (Basel) 2021; 9: 355. https://doi.org/10.3390/vaccines9040355 DOI: https://doi.org/10.3390/vaccines9040355

Martini V, Bernardi S, Russo V, Guccione J, Comazzi S, Roperto S. Blood lymphocyte subpopulations in healthy water buffaloes (Bubalus bubalis, Mediterranean lineage): Reference intervals and influence of age and reproductive history. Vet Immunol Immunopathol 2019; 211: 58-63. https://doi.org/10.1016/j.vetimm.2019.04.007 DOI: https://doi.org/10.1016/j.vetimm.2019.04.007

Guzman E, Price S, Poulsom H, Hope J. Bovine γδ T cells: cells with multiple functions and important roles in immunity. Vet Immunol Immunopathol 2012; 148: 161-167. https://doi.org/10.1016/j.vetimm.2011.03.013 DOI: https://doi.org/10.1016/j.vetimm.2011.03.013