Molecular Characterisation of an Arcanobacterium sp. Isolate from a Buffalo (Bubalus bubalis)

Siti Gusti  Ningrum; Antonia  Kreitlow; Christoph  Lämmler; Geoffrey  Foster; Maria  Borowiak; Peter  Wragg; Madeleine  Plötz; Amir  Abdulmawjood

doi:10.6000/1927-520X.2024.13.17

Authors

Siti Gusti Ningrum Universitas Wijaya Kusuma Surabaya, Faculty of Veterinary Medicine, Jl. Dukuh Kupang XXV No.54, Dukuh Kupang, Kec. Dukuhpakis, Surabaya, 60225, Indonesia
Antonia Kreitlow Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany
Christoph Lämmler Institute of Hygiene and Infectious Diseases of Animals, Justus-Liebig-Universität Giessen, Frankfurter Straße 85-89, 35392 Gießen, Germany
Geoffrey Foster SRUC Veterinary Services, RAVIC, 9 Inverness Campus, Inverness IV2 5NA, United Kingdom
Maria Borowiak German Federal Institute for Risk Assessment (BfR), Department for Biological Safety, 12277 Berlin, Germany
Peter Wragg Animal Health and Veterinary Laboratories Agency, Penrith Regional Laboratory, Merrythought, Calthwaite, Penrith CA11 9RR, UK
Madeleine Plötz Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany
Amir Abdulmawjood Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany https://orcid.org/0000-0001-5189-8381

DOI:

https://doi.org/10.6000/1927-520X.2024.13.17

Keywords:

Arcanobacterium sp., buffalo, 16S dRNA, ISR, rpoB, gap

Abstract

The objective of the current investigation was to provide a comprehensive characterisation of an Arcanobacterium isolate derived from Bubalus bubalis. The confirmation of the species identity of Arcanobacterium sp. 15/M226/2/9 in this study was achieved by examining phenotypic characteristics and phylogenetic analysis. These analyses involved determining hemolysis on Columbia sheep blood agar and biochemical parameters using the Api-Coryne test kit, MALDI-TOF MS, and partial sequencing of the universal gene encompassing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), and the two housekeeping genes rpoB and gap. Species classification based on sequencing of the 16S rRNA gene and the ISR revealed a sequence identity of 98.7% each between Arcanobacterium sp. 15/M226/2/9 and A. bovis reference strain C605018/01/1^T. In addition, for the other target genes, rpoB and gap, the concordance was 96.6% and 98.8%, respectively. The present study showed that 15/M226/2/9 isolated from B. bubalis could be a novel species.

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