Malondialdehyde as a Prognosticating Marker for Staging of HIE - A Prospective Cohort Study

Authors

  • Lavanya Pidikiti BLDE (DU) Shri B M Patil Medical College Hospital and Research Centre, Vijayapura, India
  • Anilkumar Sajjan BLDE (DU) Shri B M Patil Medical College Hospital and Research Centre, Vijayapura, India
  • Raghavendra H. Gobbur BLDE (DU) Shri B M Patil Medical College Hospital and Research Centre, Vijayapura, India https://orcid.org/0000-0002-1090-3735
  • Siddu Charki BLDE (DU) Shri B M Patil Medical College Hospital and Research Centre, Vijayapura, India
  • Vijayakumar Biradar BLDE (DU) Shri B M Patil Medical College Hospital and Research Centre, Vijayapura, India
  • M.M. Patil BLDE (DU) Shri B M Patil Medical College Hospital and Research Centre, Vijayapura, India

DOI:

https://doi.org/10.6000/1929-4247.2024.13.01.3

Keywords:

Malondialdehyde, asphyxia, cord blood, resuscitation, oxidative stress

Abstract

Introduction: Prediction of outcomes of asphyxiated neonates is imperative. Timely intervention and effective resuscitation are beneficial for intact neurodevelopmental outcomes. In this study, we assessed the level of oxidative stress at birth in cord blood and correlated it with the Hypoxic Ischemic Encephalopathy (HIE) staging and outcomes.

Methodology: This Prospective Cohort Study was conducted between January 2020 and June 2022. All neonates requiring resuscitation at birth were enrolled in a study group. The control group included neonates who did not require resuscitation. Cord blood was collected at birth, centrifuged, serum separated, and stored at -200C. Malondialdehyde (MDA) was read at 535nm on spectrometry.

Results: 102 neonates were enrolled, among which 29 neonates were asphyxiated and 73 non-asphyxiated. The cord blood mean MDA level was significantly high in Severe HIE, with a p-value of <0.001. The serum MDA level in cord blood had a significant difference in asphyxiated neonates (8.59±1.99) and normal neonates (3.18±1.04) (P<0.001). There is a significant difference in MDA levels in cord blood; the mean MDA level was significantly higher in HIE III (10.93±2.50), compared to HIE II (9.98±2.34) and HIE I(7.72±1.09) with a p-value of <0.001. Deceased neonates had higher MDA levels than those neonates who survived.

Conclusion: Neonates with high oxidative stress at birth require advanced resuscitation. MDA levels above 7.64 mm/L have 100% sensitivity and 81.4% specificity with respect to mortality in asphyxiated neonates. Hence, cord blood MDA can be a prognostic marker of oxidative stress to predict the outcomes in asphyxiated neonates.

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Published

2024-03-25

How to Cite

Pidikiti, L. ., Sajjan, A. ., Gobbur, R. H. ., Charki, S. ., Biradar, V. . ., & Patil, M. . (2024). Malondialdehyde as a Prognosticating Marker for Staging of HIE - A Prospective Cohort Study. International Journal of Child Health and Nutrition, 13(1), 25–30. https://doi.org/10.6000/1929-4247.2024.13.01.3

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Section

General Articles