Pomegranate Juice Improves Iron Status and Ameliorates Iron Deficiency Induced Cellular Changes in Saccharomyces cerevisiae

Authors

  • S.P. Balasubramani School of Life Sciences, Trans-Disciplinary University (TDU), Foundation for Revitalisation of Local Health Traditions (FRLHT), 74/2, Jarakabande Kaval, Attur PO, Yelahanka Via, Bangalore, Karnataka, India
  • G. Padmagiri School of Life Sciences, Trans-Disciplinary University (TDU), Foundation for Revitalisation of Local Health Traditions (FRLHT), 74/2, Jarakabande Kaval, Attur PO, Yelahanka Via, Bangalore, Karnataka, India
  • P. Venkatasubramanian Trans-Disciplinary University (TDU)
  • R. Vidyashankar Indian Institute of Science (IISc), Bangalore, India
  • A. Godbole School of Life Sciences, Trans-Disciplinary University (TDU), Foundation for Revitalisation of Local Health Traditions (FRLHT), 74/2, Jarakabande Kaval, Attur PO, Yelahanka Via, Bangalore, Karnataka, India

DOI:

https://doi.org/10.6000/1929-5634.2015.04.03.5

Keywords:

Iron deficiency anemia, Ayurveda, Pandu, Pomegranate, Rasayana, S. cerevisiae

Abstract

Background: Iron Deficiency Anemia (IDA) is most prevalent form of anemia affecting around 2 billion people world-wide. Ayurveda, an Indian system of medicine, describes pomegranate (Punica granatum) fruits as a Rasayana and a dietary supplement for managing a condition called Pandu, which is akin to IDA. Rasayanas are methods to maintain homeostasis by improving digestion, metabolism and absorption of nutrients and elimination of waste. Yeast (Saccharomyces cerevisiae) has been a well-accepted model organism to study iron metabolism.

Materials & Methods: In the current study we developed ‘anemic yeast’ by culturing yeast cells in iron-free medium with bathophenanthroline disulfonate (BPS). The effect of pomegranate juice (PJ) on reversing the ‘IDA like’ condition in yeast was studied.

Results: Culturing iron deficient (ID) cells in the presence of 10% PJ supplemented medium (IDP), improved iron status by at least 7 fold (p<0.0001) and reversed mitochondrial degeneration induced by iron deficiency. Percentage of healthy reticulate mitochondria in IDP cells was >30% higher (p<0.0001) than that in the ID cells grown in iron deficient medium (IDD) and at least 14% more than that in ID cells grown in 10% PJ-equivalent iron substituted media. Interestingly, PJ substitution improved the functional ferrous (Fe2+) form as well as the bio-assimilated heme form of iron, but not the ferric (Fe3+) storage form in ID cells.

Conclusion: Yeast model can be useful as a quick screen to identify potential nutritional supplements. Pomegranate’s potential role as a nutritional supplement in IDA management and as a hematinic is worthy of further research

Author Biography

P. Venkatasubramanian, Trans-Disciplinary University (TDU)

School of Life Sciences, Foundation for Revitalisation of Local Health Traditions (FRLHT)

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Published

2015-10-28

How to Cite

Balasubramani, S., Padmagiri, G., Venkatasubramanian, P., Vidyashankar, R., & Godbole, A. (2015). Pomegranate Juice Improves Iron Status and Ameliorates Iron Deficiency Induced Cellular Changes in Saccharomyces cerevisiae. Journal of Nutritional Therapeutics, 4(3), 104–112. https://doi.org/10.6000/1929-5634.2015.04.03.5

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