Cellulase and Xylanase Production from Rice Straw by a Locally Isolated Fungus Aspergillus fumigatus NITDGPKA3 under Solid State Fermentation – Statistical Optimization by Response Surface Methodology
DOI:
https://doi.org/10.6000/1929-6002.2012.01.01.7Keywords:
Cellulase, xylanase, solid state fermentation, statistical optimizationAbstract
Alkali pretreated rice straw was used as substrate for cellulase production by a locally isolated fungus Aspergillus fumigatus NITDGPKA3 under solid state fermentation. Critical process parameters such as incubation period, temperature, basal medium content and pH were statistically optimized for an enhanced cellulase and xylanase yield by response surface methodology. The design predicted an optimum yield of 3.1 IU/g dry substrate, 64.18 IU/g dry substrate and 1040.57 IU/g dry substrate for FPase, CMCase and xylanase respectively under the optimum conditions of incubation period of 90 h, temperature at 33oC, initial basal medium content of 62% and initial pH 4. The experimental values under optimum conditions correlated well with the predicted results. Further, crude enzyme extract from Aspergillus fumigatus NITDGPKA3 was used for saccharification of pretreated rice straw and this released 189.50 mg/g of reducing sugar. This work was carried out in the Department of Biotechnology, National Institute of Technology, Durgapur-713209, West Bengal, India, during the period 2010 to 2011.
References
Jeya M, Zhang YW, Kim IW, Lee JK. Enhanced saccharification. Bioresour Technol 2009; 100: 5155-61. http://dx.doi.org/10.1016/j.biortech.2009.05.040 DOI: https://doi.org/10.1016/j.biortech.2009.05.040
Sarkar N, Ghosh SK, Bannerjee S, Aikat K. Bioethanol production. Renew Ener 2012; 37: 19-27. http://dx.doi.org/10.1016/j.renene.2011.06.045 DOI: https://doi.org/10.1016/j.renene.2011.06.045
Gutierrez-Correa M, Tengerdy RP. Production of cellulase. Biotechnol Lett 1997; 19: 665-7. http://dx.doi.org/10.1023/A:1018342916095 DOI: https://doi.org/10.1023/A:1018342916095
Cen P, Xia L. Production of cellulase. Adv Biochem Eng Biotechnol 1999; 65: 69-92. (as cited in Lever M, Ho G, Cord-Ruwisch R. Ethanol from lignocellulose using crude unprocessed cellulose from solid-state fermentation. Bioresour Technol 2010; 101: 7083-7087).
Ghildyal B, Exoenzymes N. In: Doelle H, Mitchell D, Rolz C, Eds. Solid Substrate Cultivation. Chapter 11. London: Elsevier App Sci 1993. (as cited in Lever M, Ho G, Cord-Ruwisch R. Ethanol from lignocellulose using crude unprocessed cellulose from solid-state fermentation. Bioresour Technol 2010; 101: 7083-7087). DOI: https://doi.org/10.1016/j.biortech.2010.04.012
Wati L, Kumari S, Kundu BS. Paddy straw. Ind J Microbiol 2007; 47: 26-29. DOI: https://doi.org/10.1007/s12088-007-0005-y
Ghose TK. Measurement of cellulase activities. Pure Appl Chem 1987; 59: 257-68. http://dx.doi.org/10.1351/pac198759020257 DOI: https://doi.org/10.1351/pac198759020257
Aneja KR. Experiments in microbiology, plant pathology and biotechnology. 4th ed. India: New Age 2003.
Bo¨rjesson J, Peterson R, Tjerneld F. Enhanced Enzymatic Conversion. Enz Microbial Technol 2007; 40: 754-62. http://dx.doi.org/10.1016/j.enzmictec.2006.06.006 DOI: https://doi.org/10.1016/j.enzmictec.2006.06.006
Reese ET, Manguire A. Surfactants. Appl Microbiol 1969; 17: 242-5. DOI: https://doi.org/10.1128/am.17.2.242-245.1969
Ellaiah P, Adinarayana K, Bhavani Y, Padmaja P, Srinivasulu B. Optimization of process parameters. Proc Biochem 2002; 38: 615-20. http://dx.doi.org/10.1016/S0032-9592(02)00188-7 DOI: https://doi.org/10.1016/S0032-9592(02)00188-7
Feniksova RV, Tikhomirova AS, Rakhleeva EE. Conditions for forming amylase. Microbiologia 1960; 29: 745-8.
Lonsane BK, Ghildyal NP, Budiatman S, Ramkrishna SV. Engineering aspects of solid state fermentation. Enzyme Microbial Technol 1985; 7: 258-65. http://dx.doi.org/10.1016/0141-0229(85)90083-3 DOI: https://doi.org/10.1016/0141-0229(85)90083-3
Zadrazil F, Brunnert H. Investigation of physical parameters. Eur J Appl Microbiol Biotechnol 1981; 11: 183-8. DOI: https://doi.org/10.1007/BF00511259
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2012 Journal of Technology Innovations in Renewable Energy
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Policy for Journals/Articles with Open Access
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work
Policy for Journals / Manuscript with Paid Access
Authors who publish with this journal agree to the following terms:
- Publisher retain copyright .
- Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work .