Unlocking the Potential of Multiple Ovulation and Embryo Transfer (MOET) in Water Buffaloes

Ronald  Nuwasasira; Peregrino G.  Duran; Danilda  Hufana-Duran

doi:10.6000/1927-520X.2026.15.02

Authors

Ronald Nuwasasira Department of Animal Science, Central Luzon State University, Science City of Munoz, Nueva Ecija 3120, Philippines and School of Agriculture and Applied Sciences, Bugema University, Kampala 6529, Uganda
Peregrino G. Duran Department of Animal Science, Central Luzon State University, Science City of Munoz, Nueva Ecija 3120, Philippines and Reproduction and Physiology Section, Research and Development Division, Department of Agriculture-Philippine Carabao Center, Science City of Munoz, Nueva Ecija 3120, Philippines
Danilda Hufana-Duran Department of Animal Science, Central Luzon State University, Science City of Munoz, Nueva Ecija 3120, Philippines and Reproduction and Physiology Section, Research and Development Division, Department of Agriculture-Philippine Carabao Center, Science City of Munoz, Nueva Ecija 3120, Philippines

DOI:

https://doi.org/10.6000/1927-520X.2026.15.02

Keywords:

ART, MOET, embryo collection, reproductive biotechnology, superovulation

Abstract

Water buffaloes (Bubalus bubalis) are essential to agricultural economies across Asia and other regions, providing milk, meat, and draught power. Despite their importance, reproductive efficiency remains lower than in cattle due to species-specific constraints, including limited ovarian reserves, poor superovulatory responses, and suboptimal uterine conditions. Multiple Ovulation and Embryo Transfer (MOET) offers a promising strategy for accelerating genetic improvement, particularly in swamp buffalo-dominated areas seeking the infusion of riverine germplasm. While MOET is widely successful in cattle, its application in buffaloes is challenged by anatomical, endocrine, and physiological differences. This review synthesizes current knowledge on MOET development in water buffaloes, highlighting historical milestones, reproductive limitations, protocol refinements, hormonal strategies, embryo recovery innovations, and the role of international collaborations. By addressing biological and logistical barriers, MOET can become a strategic tool to enhance genetic gain, improve reproductive outcomes, and support sustainable buffalo production systems.

References

Borghese A. Situation and perspectives of buffalo in the world, Europe, and Macedonia. Maced Anim Sci J 2011; 1(2): 281-296. DOI: https://doi.org/10.54865/mjas112281b

Minervino AHH, Zava M, Vecchio D, Borghese A. Bubalus bubalis: A short story. Front Vet Sci 2020; 7: 570413. DOI: https://doi.org/10.3389/fvets.2020.570413

Naveena BM, Kiran M. Buffalo meat quality, composition, and processing characteristics: Contribution to the global economy and nutritional security. Anim Front 2014; 4(4): 18-24. DOI: https://doi.org/10.2527/af.2014-0029

Nava-Trujillo H, Valeris-Chacin R, Morgado-Osorio A, Zambrano-Salas S, Tovar-Breto L, Quintero-Moreno A. Reproductive performance of water buffalo cows: A review of affecting factors. J Buffalo Sci 2020; 9: 133-151. DOI: https://doi.org/10.6000/1927-520X.2019.08.03.15

Drost M, Alexiev A, Vlahov K, Karaivanov C, Cripe WS, Leonards AP, et al. Successful nonsurgical embryo transfer in buffalo (Bubalus bubalis) in Bulgaria. Theriogenology 1988; 30(4): 659-668. DOI: https://doi.org/10.1016/0093-691X(88)90301-9

Drost M, Wright JM Jr, Cripe W, Richter AR. Embryo transfer in water buffalo (Bubalus bubalis). Theriogenology 1983; 20(5): 579-584. DOI: https://doi.org/10.1016/0093-691X(83)90082-1

Srirattana K, Hufana-Duran D, Atabay AP, Duran PG, Atabay EC, Lu K, et al. Current status of assisted reproductive technologies in buffaloes. Anim Sci J 2022; 93: e13767. DOI: https://doi.org/10.1111/asj.13767

Drost M. Advanced reproductive technology in the water buffalo. Theriogenology 2007; 68(3): 450-453. DOI: https://doi.org/10.1016/j.theriogenology.2007.04.013

Beg MA, Sanwal PC, Yadav MC. Ovarian response and endocrine changes in buffalo superovulated at midluteal and late luteal stage of the estrous cycle: A preliminary report. Theriogenology 1997; 47: 423. DOI: https://doi.org/10.1016/S0093-691X(97)00001-0

Bhat GR, Dhaliwal GS. Estrus and ovulation synchrony of buffaloes (Bubalus bubalis): A review. Buffalo Bull 2023; 42(2): 2415. DOI: https://doi.org/10.56825/bufbu.2023.4222415

Baruselli PS, De Carvalho NA, Gasparrini B, Campanile G, D'Occhio MJ. Development, adoption, and impact of assisted reproduction in domestic buffaloes. Anim 2023; 17: 100764. DOI: https://doi.org/10.1016/j.animal.2023.100764

Hufana-Duran D, Pedro PB, Venturina HV, Hufana RD, Salazar AL Jr, Duran PG, et al. Post-warming hatching and birth of live calves following transfer of in vitro-derived vitrified water buffalo (Bubalus bubalis) embryos. Theriogenology 2004; 61: 1429-1439. DOI: https://doi.org/10.1016/j.theriogenology.2003.08.011

Hufana-Duran D, Pedro PB, Salazar AL Jr, Venturina HV, Duran PG, Cruz LC. River buffalo calves (2n=50) delivered to term by swamp buffalo recipients (2n=48) out of in vitro-derived vitrified embryos. Livest Sci 2007; 107: 213-219. DOI: https://doi.org/10.1016/j.livsci.2006.09.022

Hufana-Duran D, Pedro PB, Salazar AL Jr, Venturina HV, Duran PG, Takahashi Y, et al. Twin calf production in water buffaloes following non-surgical transfer of in vitro-produced vitrified embryos. Philipp J Sci 2008; 137(2): 99-104.

Singla SK, Singh B. Multiple ovulation and embryo transfer in livestock production. In: Kumaresan A, Srivastava AK, editors. Frontier technologies in bovine reproduction. Singapore: Springer; 2022. DOI: https://doi.org/10.1007/978-981-19-3072-0_10

Callesen H, Liboriussen T, Greve T. Practical aspects of multiple ovulation-embryo transfer in cattle. Anim Reprod Sci 1996; 42: 215-226. DOI: https://doi.org/10.1016/0378-4320(96)01513-8

Hufana-Duran D, Duran PG. Advanced reproductive technologies in water buffalo. In: Purohit GN, editor. Bubaline theriogenology. Ithaca (NY): International Veterinary Information Service; 2015. Available from: https://www.ivis. org/library/bubaline-theriogenology/advanced-reproductive-technologies-water-buffalo

Baruselli PS, Soares JG, Bayeux BM, Silva JC, Mingoti RD, Carvalho NA. Assisted reproductive technologies (ART) in water buffaloes. Anim Reprod 2018; 15(Suppl 1): 971. DOI: https://doi.org/10.21451/1984-3143-AR2018-0043

Palanisammi A, Satheshkumar S, Rangasamy S. Super-ovulatory response and embryo yield in buffaloes (Bubalus bubalis). J Entomol Zool Stud 2020; 8(6): 1468-1470.

Marin DFD, de Souza EB, de Brito VC, Nascimento CV, Ramos AS, Filho STR, et al. In vitro embryo production in buffaloes: From the laboratory to the farm. Anim Reprod 2019; 16(2): 260-266. DOI: https://doi.org/10.21451/1984-3143-AR2018-0135

Coman S, Berean DI, Cimpean R, Ciupe S, Coman I, Bogdan LM. Clinical modalities for enhancing reproductive efficiency in buffaloes: A review. Anim 2024; 14(18): 2642. DOI: https://doi.org/10.3390/ani14182642

Baruselli PS, Carvalho JGSD, Elliff FM, Silva JCBD, Chello D, Carvalho NATD. Embryo transfer in buffalo (Bubalus bubalis). Theriogenology 2020; 150: 221-228. DOI: https://doi.org/10.1016/j.theriogenology.2020.01.037

Carvalho NA, Baruselli PS, Zicarelli L, Madureira EH, Visintin JA, D'Occhio MJ. Control of ovulation with a GnRH agonist after superstimulation of follicular growth in buffalo: Fertilization and embryo recovery. Theriogenology 2002; 58(9): 1641-1650. DOI: https://doi.org/10.1016/S0093-691X(02)01057-9

Campanile G, Baruselli PS, Neglia G, Vecchio D, Gasparrini B, Gimenes LU, et al. Ovarian function in the buffalo and implications for embryo development and assisted reproduction. Anim Reprod Sci 2010; 121(1-2): 1-11. DOI: https://doi.org/10.1016/j.anireprosci.2010.03.012

Bertoni A, Napolitano F, Mota-Rojas D, Sabia E, Álvarez-Macías A, Mora-Medina P, et al. Similarities and differences between river buffaloes and cattle: Health, physiological, behavioral and productivity aspects. J Buffalo Sci 2020; 9: 92-109. DOI: https://doi.org/10.6000/1927-520X.2020.09.12

Baruselli PS, Ferreira RM, Sales JF, Gimenes LU, Sá Filho MF, Martins CM, et al. Timed embryo transfer programs for management of donor and recipient cattle. Theriogenology 2011; 76(9): 1583-1593. DOI: https://doi.org/10.1016/j.theriogenology.2011.06.006

De Rensis F, Garcia-Ispierto I, López-Gatius F. Seasonal heat stress: Clinical implications and hormone treatments for the fertility of dairy cows. Theriogenology 2015; 84(5): 659-666. DOI: https://doi.org/10.1016/j.theriogenology.2015.04.021

Ty LV, Chupin D, Driancourt MA. Ovarian follicular populations in buffaloes and cows. Anim Reprod Sci 1989; 19(3-4): 171-178. DOI: https://doi.org/10.1016/0378-4320(89)90090-0

Carvalho NAT, Soares JG, Kahwage PR, Garcia AR. Anatomy of the reproductive tract of the female and male buffaloes. In: Purohit GN, editor. Bubaline theriogenology. Ithaca (NY): International Veterinary Information Service; 2014. Available from: https://www.ivis.org/library/bubaline-theriogenology/anatomy-of-reproductive-tract-of-female-and-male-buffaloes

Dobson H, Kamonpatana M. A review of female cattle reproduction with special reference to a comparison between buffaloes, cows and zebu. J Reprod Fertil 1986; 77(1): 1-36. DOI: https://doi.org/10.1530/jrf.0.0770001

Drost M. Training manual for embryo transfer in buffalo. Rome: Food and Agriculture Organization of the United Nations; 1991.

Baruselli PS, Madureira EH, Visintin JA, Porto-Filho R, Carvalho NAT, Campanile G, et al. Failure of oocyte entry into oviduct in superovulated buffalo. Theriogenology 2000; 53(1): 491.

Baruselli PS, Soares JG, Gimenes LU, Monteiro BM, Olazarri MJ, Carvalho NATD. Control of buffalo follicular dynamics for artificial insemination, superovulation, and in vitro embryo production. Buffalo Bull 2013; 32(1): 160-167.

Singh B, Chauhan MS, Singla SK, Gautam SK, Verma V, Manik RS, et al. Reproductive biotechniques in buffaloes (Bubalus bubalis): Status, prospects and challenges. Reprod Fertil Dev 2009; 21(4): 499-510. DOI: https://doi.org/10.1071/RD08172

Neglia G, Bifulco G. Multiple ovulation and embryo transfer in the buffalo species. In: The buffalo (Bubalus bubalis): Production and research. Bentham Science Publishers; 2017. p. 340-373. DOI: https://doi.org/10.2174/9781681084176117010016

Cruz LC, Venturina H, Jha SS, Adriano F, Serra P, Duran PG, et al. Successful transfer of Murrah buffalo embryo into Philippine swamp buffalo recipients. In: Proceedings of the 3rd World Buffalo Congress; 1991; Varna, Bulgaria. p. 586-590.

Misra A, Tyagi S. In vivo embryo production in buffalo: Present and perspectives. Ital J Anim Sci 2007; 6(Suppl 2): 74-91.

Ohashi OM, Kumar S, Rolim Filho ST, Ribeiro HFL, Freitas VJDF, Vale WG. Advances in embryo production in buffaloes: In vivo versus in vitro procedures. In: Chauhan MS, Selokar N, editors. Biotechnological applications in buffalo research. Singapore: Springer; 2022. DOI: https://doi.org/10.1007/978-981-16-7531-7_14

Mapletoft RJ, Bó GA. Innovative strategies for superovulation in cattle. Anim Reprod 2012; 10(3): 174-179.

Abd-Allah SM, Sharma RK, Phulia SK, Singh I. Effect of repeated dual superovulation using FSH and PMSG+FSH on ovulatory response in Murrah buffaloes. Theriogenology Insight 2014; 4(2): 117-124. DOI: https://doi.org/10.5958/2277-3371.2014.00734.7

Gasparrini B. In vivo embryo production in buffalo: current situation and future perspectives. Ital J Anim Sci 2007; 6(Suppl 2): 74-91. DOI: https://doi.org/10.4081/ijas.2007.s2.74

Perry G, Salverson R. Understanding estrus synchronization of cattle. SDSU Extension 2020; P-00169. Available from: https://extension.sdstate.edu/sites/default/files/2020-09/P-00169.pdf

Purohit GN, Pankaj T, Munesh P, Mitesh G, Chandra SS, Atul SA, et al. Estrus synchronization in buffaloes: prospects, approaches and limitations. Pharma Innov J 2019; 8(2): 54-62. Available from: https://www.thepharmajournal.com/ archives/2019/vol8issue2/PartB/7-12-25-308.pdf

Neglia G, Gasparrini B, Di Palo R, De Rosa C, Zicarelli L, Campanile G. Comparison of pregnancy rates with two estrus synchronization protocols in Italian Mediterranean buffalo cows. Theriogenology 2003; 60(1): 125-133. DOI: https://doi.org/10.1016/S0093-691X(02)01328-6

Dharani S, Kathiresan D, Devanathan TG, Balachandran C, Satheshkumar S. Ovulatory response of first follicular wave growing and regressing phase follicle for GnRH adminis-tration in cyclic buffaloes. Buffalo Bull 2010; 29(3): 199-205.

Gautam G, Rokaya S, Devkota B, Sapkota S. Seasonality of reproduction in buffaloes in subtropical regions of Southern Nepal. Adv Anim Vet Sci 2024; 12(5): 895-901. DOI: https://doi.org/10.17582/journal.aavs/2024/12.5.895.901

Martínez CA, Nohalez A, Parrilla I, Vázquez JL, Roca J, Cuello C, et al. Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows. Theriogenology 2017; 87: 316-320. DOI: https://doi.org/10.1016/j.theriogenology.2016.09.009

Misra A, Kasiraj R, Mutha RM, Rangareddy N, Jaiswal R, Pant H. Rate of transport and development of preimplantation embryo in the superovulated buffalo (Bubalus bubalis). Theriogenology 1998; 50(4): 637-649.

Misra A, Kasiraj R, Mutha RM, Rangareddy N, Jaiswal R, Pant H. Rate of transport and development of preimplantation embryo in the superovulated buffalo (Bubalus bubalis). Theriogenology 1998; 50(4): 637-649. DOI: https://doi.org/10.1016/S0093-691X(98)00168-X

Binelli M, Martins T, Arthington J, Waters K, Mercadante V, Moriel P, et al. Practical uses for ultrasound in managing beef cattle reproduction. EDIS Publ 2020; AN113.

Hasler JF. The current status of oocyte recovery, in vitro embryo production, and embryo transfer in domestic animals, with an emphasis on the bovine. J Anim Sci 1998; 76(Suppl 3): 52-74. DOI: https://doi.org/10.2527/1998.76suppl_352x

Terzano GM. Ultrasonography and reproduction in buffalo. J Buffalo Sci 2012; 1(2). DOI: https://doi.org/10.6000/1927-520X.2012.01.02.06

Pugliesi G, de Melo GD, Ataíde GA Jr, Pellegrino CAG, Silva JB, Rocha CC, et al. Use of Doppler ultrasonography in embryo transfer programs: feasibility and field results. Anim Reprod 2018; 15(3): 239-246. DOI: https://doi.org/10.21451/1984-3143-AR2018-0059

Karaivanov C, Kacheva D, Petrov M, Vlahov K, Sapundjiev E. Superovulatory response of river buffalo (Bubalus bubalis). Theriogenology 1990; 33(2): 453-464. DOI: https://doi.org/10.1016/0093-691X(90)90503-L

Li X, Wang YL, Chen J. Infectious diseases in water buffalo: a review of current control strategies. Int J Mol Vet Res 2024; 14(6): 227-234. DOI: https://doi.org/10.5376/ijmvr.2024.14.0026

Perera GD, Pushpakumara PG, Alexander PA. Production, cryopreservation, and transfer of Murrah embryos through multiple ovulation and embryo transfer (MOET) technology in Sri Lanka. Explor Anim Med Res 2024; 14(1). Available from: https://animalmedicalresearch.org/Vol.14_Issue-1_June_2024/Production,%20cryopreservation,%20and%20transfer%20of%20murrah%20embryos.pdf

Singhal S, Prasad S, Singh H, Shukla M, Prasad JK. Effects of pre-treatment with GnRH on the efficiency of superstimulatory protocol in water buffalo (Bubalus bubalis). Iran J Appl Anim Sci 2021; 11(2).

Singh N, Dhaliwal GS, Malik VS, Dadarwal D, Honparkhe M, Singhal S, et al. Comparison of follicular dynamics, superovulatory response, and embryo recovery between estradiol-based and conventional superstimulation protocol in buffaloes (Bubalus bubalis). Vet World 2015; 8(8): 983-988. DOI: https://doi.org/10.14202/vetworld.2015.983-988

Patel DV, Singh SP, Shukla HR, Devanand CP, Kasiraj R. Superovulatory response to FSH and embryo recovery rate in Pandharpuri buffaloes (Bubalus bubalis). Buffalo Bull 2010; 29(4): 244-255.

Zambrano-Varón JL, Bon Durant RH. Superovulatory response of water buffalo (Bubalus bubalis) to gonadotrophins out of the breeding season. Ital J Anim Sci 2007; 6(Suppl 2): 626-628. DOI: https://doi.org/10.4081/ijas.2007.s2.626

Techakumphu M, Sukavong Y, Intaramongkol S, Intaramongkol J. The effect of gonadotropin-releasing hormone on superovulation in elite swamp buffalo cows (Bubalus bubalis). J Vet Med Sci 2001; 63(8): 853-857. DOI: https://doi.org/10.1292/jvms.63.853

Baruselli PS, Madureira EH, Barnabe VH, Barnabe RC, Berber RC. Evaluation of synchronization of ovulation for fixed-timed insemination in buffalo (Bubalus bubalis). Braz J Vet Res Anim Sci 2003; 40: 431-442. DOI: https://doi.org/10.1590/S1413-95962003000600007

Redhead A, Siew N, Lambie N, Carnarvon D, Ramgattie R, Knights M. The relationship between circulating concentration of AMH and LH content in follicle-stimulating hormone (FSH) preparations on follicular growth and ovulatory response to superovulation in water buffaloes. Anim Reprod Sci 2017; 188: 66-73. DOI: https://doi.org/10.1016/j.anireprosci.2017.11.010

Currin L, Baldassarre H, de Macedo MP, Glanzner WG, Gutierrez K, Lazaris K, et al. Optimization of gonadotropin stimulation protocols for in vitro embryo production in prepubertal Mediterranean water buffalo. Theriogenology 2023; 197: 84-93. DOI: https://doi.org/10.1016/j.theriogenology.2022.11.043

Pasha MMH, Moniruzzaman M, Islam MN, Alam MH, Das SK. Estrus synchronization of native buffaloes using PGF2α and GnRH-based protocols in Bangladesh. Bangladesh J Livest Res 2021; 28(1): 50-60. DOI: https://doi.org/10.3329/bjlr.v28i1.72057

Karaivanov C. Comparative studies on the superovulatory effect of PMSG and FSH in water buffalo (Bubalus bubalis). Theriogenology 1986; 26(1): 51-59. DOI: https://doi.org/10.1016/0093-691X(86)90111-1

Ocampo MB, Ocampo LC, Rayos AA, Kanagawa H. Present status of embryo transfer in water buffalo (a review). Jpn J Vet Res 1989; 37(3-4): 167-179.

Heleil B, El-Kon I, El Deeb Y. Assessment of superovulatory response using hormonal profile in buffalo (Bubalus bubalis). Glob Vet 2010; 4(4): 337-342.

Misra A, Pant H. Estrus induction following PGF2α treatment in the superovulated buffalo (Bubalus bubalis). Theriogenology 2003; 59(5-6): 1203-1207. DOI: https://doi.org/10.1016/S0093-691X(02)01167-6

Petrovas G, Kosior MA, Presicce GA, Russo M, Zullo G, Albero G, et al. FSH stimulation with short withdrawal improves oocyte competence in Italian Mediterranean buffalo (Bubalus bubalis). Animals (Basel) 2020; 10(11): 1997. DOI: https://doi.org/10.3390/ani10111997

Salzano A, De Canditis C, Della Ragione F, Prandi A, Zullo G, Neglia G, et al. Evaluation of factors involved in the failure of ovum capture in superovulated buffaloes. Theriogenology 2018; 122: 102-108. DOI: https://doi.org/10.1016/j.theriogenology.2018.09.007

Sianturi RSG, Kusumaningrum DA, Praharani L. Ovarian dynamics and progesterone profiles during estrus cycle in swamp buffaloes. AIP Conf Proc 2023; 2628(1): 070008. DOI: https://doi.org/10.1063/5.0153945

Berisha B, Rodler D, Schams D, Sinowatz F, Pfaffl MW. Prostaglandins in superovulation-induced bovine follicles during the preovulatory period and early corpus luteum. Front Endocrinol (Lausanne) 2019; 10: 467. DOI: https://doi.org/10.3389/fendo.2019.00467

Mondal S, Prakash BS, Palta P. Endocrine aspects of oestrous cycle in buffaloes (Bubalus bubalis): an overview. Asian-Australas J Anim Sci 2006; 19(6): 789-798.

Perera BMAO. Reproductive cycles of buffalo. Anim Reprod Sci 2008; 124(3-4): 194-199. DOI: https://doi.org/10.1016/j.anireprosci.2010.08.022

Azawi OI. Etiopathology and therapy of retained fetal membranes and postpartum uterine infection in buffaloes. Theriogenology 2013; Int Vet Info Serv A5719.0413.

Jongsuwanwattana R, Swangchan-Uthai T, Sirivaidyapong S. Reproduction and in vitro technologies of swamp buffalo: past, present, and future. Thai J Vet Med 2024; 54(2): 115-124. DOI: https://doi.org/10.56808/2985-1130.3726

Paul DR, Lakhanpal D, Biswas N. Clinical management of retention of fetal membranes in Murrah buffalo. Int J Vet Sci Anim Husb 2024; 9(4): 575-577. DOI: https://doi.org/10.22271/veterinary.2024.v9.i4i.1599

Gimenes LU, Sá Filho MF, Carvalho NAT, Torres-Júnior JRS, Souza AH, Madureira EH, et al. Follicle deviation and ovulatory capacity in Bos indicus heifers treated with progesterone, estradiol, and eCG. Theriogenology 2008; 69(7): 852-858. DOI: https://doi.org/10.1016/j.theriogenology.2008.01.001

El-Sanea AM, Abdoon ASS, Kandil OM, El-Toukhy NE, Abo El-Maaty AM, Ahmed HH. Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes cultured in vitro. Vet World 2021; 14(1): 78-84. DOI: https://doi.org/10.14202/vetworld.2021.78-84

Ghanem N, Ahmed DAR, Dessouki SM, Faheem MS, Gad AY, Peippo J, et al. Cellular and molecular alterations of buffalo oocytes cultured under two different levels of oxygen tension during in vitro maturation. Zygote 2021; 29(4): 273-284. DOI: https://doi.org/10.1017/S0967199420000945

Craciunas L, Gallos I, Chu J, Bourne T, Brosens JJ. Conventional and modern markers of endometrial receptivity: a systematic review and meta-analysis. Hum Reprod Update 2019; 25(2): 202-223. DOI: https://doi.org/10.1093/humupd/dmy044

Sun B, Yeh J. Non-invasive and mechanism-based molecular assessment of endometrial receptivity during the window of implantation: current concepts and future prospective testing directions. Front Reprod Health 2022; 4: 863173. DOI: https://doi.org/10.3389/frph.2022.863173

Sun T, Shen J, Achilli A, Chen N, Chen Q, Dang R, et al. Genomic analyses reveal distinct genetic architectures and selective pressures in buffaloes. Gigascience 2020; 9(2): giz166. DOI: https://doi.org/10.1093/gigascience/giz166

van Hooft P, Dougherty ER, Getz WM, Greyling BJ, Zwaan BJ, Bastos ADS. Genetic responsiveness of African buffalo to environmental stressors: a role for epigenetics in balancing autosomal and sex chromosome interactions. PLoS One 2018; 13(2): e0191481. DOI: https://doi.org/10.1371/journal.pone.0191481

Chiariotti A, Borghese A, Boselli C, Barile VL. Water buffalo’s adoptability to different environments and farming systems: a review. Animals (Basel) 2025; 15(11): 1538. DOI: https://doi.org/10.3390/ani15111538

Maylem ERS, Ablaza IINF, Lofranco JOC, Cruz CFD, Tadeo RD, Atabay EP, et al. Use of double PGF2α in a 7-day CIDR-Synch timed artificial insemination in water buffaloes during summer. Vet Anim Sci 2025; 29: 100474. DOI: https://doi.org/10.1016/j.vas.2025.100474

El Debaky HA, Kutchy NA, Ul-Husna AA, Indriastuti R, Akhter S, Purwantara B, et al. Potential of water buffalo in world agriculture: challenges and opportunities. Appl Anim Sci 2019; 35(2): 255-268. DOI: https://doi.org/10.15232/aas.2018-01810

Hufana-Duran D, Chaikhun-Marcou T, Duran PG, Atabay EP, Nguyen HT, Atabay EC, et al. Future of reproductive biotechnologies in water buffalo in Southeast Asian countries. Theriogenology 2025; 233: 123-130. DOI: https://doi.org/10.1016/j.theriogenology.2024.11.016

Macias J, Vivanco W, Hurtado E, Larrea C, Andres J, Duran P, Hufana-Duran D. Plasma Anti-Müllerian Hormone level and its relationship to ovarian reserve, superovulation response, and embryo survival in Brahman (Bos Indicus) females. J ISSAAS 2023; 29(2): 62-77.