Sequencing and Analysis of the Myostatin Gene (GDF-8) in Bubalus bubalis Young Animals to Determine the Existence of Possible Mutations Expressed in Double Musculature Phenotype
DOI:
https://doi.org/10.6000/1927-520X.2021.10.01Keywords:
Myostatin, mutation, double muscle, muscle hyperplasia, Bubalus bubalis.Abstract
Since the 19th century, the presentation of bovines with disproportionate muscle development have been associated with mutations that inhibit the action of the myostatin gene, it is referred to as double muscle mutation, which is common in some European Bos taurus breeds but it is not reported in buffaloes Bubalus bubalis. This study aims to evaluate if the phenotype observed in 6 young buffaloes with disproportionate muscle development has the same myostatin mutation reported in cattle. DNA was obtained from the blood of the animals of the Murrah breed. First, second and third exon was amplified end point PCR; the fragments were sequenced using capillary electrophoresis. Holstein cattle (Bos taurus) was used As control for normal phenotype. The results obtained from the comparison of the sequence of the myostatin gene show that the observed double-muscled phenotype did not show differences from normal controls. Interspecific variation was demonstrated by comparing exons two and three of the gene, finding 12 variations between the Bos taurus and Bubalus bubalis species in the evaluated fragments. It is necessary to study physiology, and the animals to explain the phenotype observed in buffaloes.
References
Leipold HW, Dennis SM. Congenital defects affecting bovine reproduction. In: Morrow D.A. (Ed.), Current Therapy in Theriogenology: Diagnosis, treatment and prevention of reproductive diseases in small and large animals. W.B. Saunders Company, Philadelphia 1986; 177-199.
Jolly RD. Screening for genetic diseases in cattle. Aust Vet J 2002; 80(5): 284-285. https://doi.org/10.1111/j.1751-0813.2002.tb10843.x
Arthur PF, Makarechian M, Price MA. Incidence of dystocia and perinatal calf mortality resulting from the reciprocal crossing of double-muscled and normal cattle. Canadá: Canadian Vet Journal 1988; 29: 163-167.
Miranda M, Amigues Y, Boscher M, Menissier F, Corte's O, Dunner S, Anim J. Simultaneous genotyping to detect myostatin gene polymorphism in beef cattle breeds. Breed Genet 2002; 119: 361-366. https://doi.org/10.1046/j.1439-0388.2002.00352.x
Royo MLF. Secuenciación y análisis del gen de la miostatina bovina. Tesis Doctoral Universidad Complutense de Madrid, Facultad de Medicina Veterinaria. Madrid 2002.
Grobet L, Royo-Martin LJ, Poncelet D, Pirottin D, Brouwers B, et al. A deletion in the myostatin gene causes double-muscling in cattle. Milan, Italy: Nat Genet 1997; 17: 71-74. https://doi.org/10.1038/ng0997-71
Moreno V, Sifuentes A, De La Rosa X, Pereyra B. Evaluación de regiones polimórficas del gen de la miostatina en ganado beefmaster. Arch Med Vet 2008; 40: 59-63. https://doi.org/10.4067/S0301-732X2008000100008
Charlier C, Coppieters W, Farnir F. The mh gene causing double-muscling in cattle maps to bovine Chromosome 2. Belgica: Mamm Genome 2002.
McPherron AC, Lawler AM, Lee SJ. Regulation of skeletal muscle in mice by a new TGF-beta superfamily member. Nature 1997; 387: 83-90. https://doi.org/10.1038/387083a0
Grobet L, Poncelet D, Royo LJ, Brouwers B, Pirottin D, Michaux C, Mennissier F, Zanotti M, Dunner S, Georges M. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle. Mamm Genome 1997; 9: 210-213. https://doi.org/10.1007/s003359900727
Vermorel M, Ortigues I, Vernet J, Geay Y, Jailler R, Jailler R, Baumont R, Hocquette JF, Deswysen AG. Energy metabolism in normal and double-muscled BelgianBlue calves in relation with body composition and organ size. In Energy Metabolism of Farm Animals; Aguilera, J.F., Ed., Consejo Superior de Investigaciones: Granada, Spain 1997; pp. 209-211.
Ménissier F. General survey of the effect of double muscling on cattle performance. Curr Top In Vet Med And Anim Sci 1982; 23-53. https://doi.org/10.1007/978-94-009-7550-7_2
Mota LSLS, Curi RA, Palmieri DA, Borges AS. Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence. Genet Mol Biol 2006; 29: 79-82. https://doi.org/10.1590/S1415-47572006000100016
Marcolongo-Pereira C, Schild AL, Soares MP, Vargas Jr, SF, and Riet-Correa F. Defeitos congênitos diagnosticados em ruminantes na Região Sul do Rio Grande do Sul. Pesquisa Veterinária Brasileira 2010; 30(10): 816-826. https://doi.org/10.1590/S0100-736X2010001000003
Mota LSLS, Curi RA, Palmieri DA, Borges AS. Mutação no exon 3 do gene da miostatina de búfalos (Bubalus bubalis). Resumos do 49° Confresso Brasileiro de Genética Água de Lindóia, SP 16 a 19 de Setembro 2003. www.sgb.org.br (Resumen).
Kambadur R, Sharma M. Mutations in myostatin (GDF8) in Double-Muscled Belgian Blue and Piedmontese Cattle. Research, Ruakura, Hamilton, New Zealand; 3 US Department of Agriculture (USDA)/Agricultural Research Service (ARS). Meat Animal Research Center, Clay Center. Nebraska 1997; 68933-0166 7:910–915 ©1997. https://doi.org/10.1101/gr.7.9.910
McPherron AC, Lawer AM, Lee SJ. Double muscling in cattle due to mutations in the myostatine gene. Proc Natl Acad Sci USA 1997; 94: 12457-12461. https://doi.org/10.1073/pnas.94.23.12457
Barbosa JD, Tury E, Pfeifer Barbosa IB, Cunha Dias VR. Hiperplasia muscular congênita (Doppellender, Double Muscling, Culard) em búfalos no Estado do Pará, Brasil. Ciênc Vet Trop 1999; 2: 50-52.
Leipold HW, Dennis SM. Congenital defects affecting bovine reproduction. In: Morrow D.A. (Ed.), Current Therapy in Theriogenology: Diagnosis, treatment and prevention of reproductive diseases in small and large animals. W.B. Saunders Company, Philadelphia 1986; 177-199.
Jolly RD. Screening for genetic diseases in cattle. Aust Vet J 2002; 80(5): 284-285. https://doi.org/10.1111/j.1751-0813.2002.tb10843.x DOI: https://doi.org/10.1111/j.1751-0813.2002.tb10843.x
Arthur PF, Makarechian M, Price MA. Incidence of dystocia and perinatal calf mortality resulting from the reciprocal crossing of double-muscled and normal cattle. Canadá: Canadian Vet Journal 1988; 29: 163-167.
Miranda M, Amigues Y, Boscher M, Menissier F, Corte's O, Dunner S, Anim J. Simultaneous genotyping to detect myostatin gene polymorphism in beef cattle breeds. Breed Genet 2002; 119: 361-366. https://doi.org/10.1046/j.1439-0388.2002.00352.x DOI: https://doi.org/10.1046/j.1439-0388.2002.00352.x
Royo MLF. Secuenciación y análisis del gen de la miostatina bovina. Tesis Doctoral Universidad Complutense de Madrid, Facultad de Medicina Veterinaria. Madrid 2002.
Grobet L, Royo-Martin LJ, Poncelet D, Pirottin D, Brouwers B, et al. A deletion in the myostatin gene causes double-muscling in cattle. Milan, Italy: Nat Genet 1997; 17: 71-74. https://doi.org/10.1038/ng0997-71 DOI: https://doi.org/10.1038/ng0997-71
Moreno V, Sifuentes A, De La Rosa X, Pereyra B. Evaluación de regiones polimórficas del gen de la miostatina en ganado beefmaster. Arch Med Vet 2008; 40: 59-63. https://doi.org/10.4067/S0301-732X2008000100008 DOI: https://doi.org/10.4067/S0301-732X2008000100008
Charlier C, Coppieters W, Farnir F. The mh gene causing double-muscling in cattle maps to bovine Chromosome 2. Belgica: Mamm Genome 2002.
McPherron AC, Lawler AM, Lee SJ. Regulation of skeletal muscle in mice by a new TGF-beta superfamily member. Nature 1997; 387: 83-90. https://doi.org/10.1038/387083a0 DOI: https://doi.org/10.1038/387083a0
Grobet L, Poncelet D, Royo LJ, Brouwers B, Pirottin D, Michaux C, Mennissier F, Zanotti M, Dunner S, Georges M. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle. Mamm Genome 1997; 9: 210-213. https://doi.org/10.1007/s003359900727 DOI: https://doi.org/10.1007/s003359900727
Vermorel M, Ortigues I, Vernet J, Geay Y, Jailler R, Jailler R, Baumont R, Hocquette JF, Deswysen AG. Energy metabolism in normal and double-muscled BelgianBlue calves in relation with body composition and organ size. In Energy Metabolism of Farm Animals; Aguilera, J.F., Ed., Consejo Superior de Investigaciones: Granada, Spain 1997; pp. 209-211.
Ménissier F. General survey of the effect of double muscling on cattle performance. Curr Top In Vet Med And Anim Sci 1982; 23-53. https://doi.org/10.1007/978-94-009-7550-7_2 DOI: https://doi.org/10.1007/978-94-009-7550-7_2
Mota LSLS, Curi RA, Palmieri DA, Borges AS. Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence. Genet Mol Biol 2006; 29: 79-82. https://doi.org/10.1590/S1415-47572006000100016 DOI: https://doi.org/10.1590/S1415-47572006000100016
Marcolongo-Pereira C, Schild AL, Soares MP, Vargas Jr, SF, and Riet-Correa F. Defeitos congênitos diagnosticados em ruminantes na Região Sul do Rio Grande do Sul. Pesquisa Veterinária Brasileira 2010; 30(10): 816-826. https://doi.org/10.1590/S0100-736X2010001000003 DOI: https://doi.org/10.1590/S0100-736X2010001000003
Mota LSLS, Curi RA, Palmieri DA, Borges AS. Mutação no exon 3 do gene da miostatina de búfalos (Bubalus bubalis). Resumos do 49° Confresso Brasileiro de Genética Água de Lindóia, SP 16 a 19 de Setembro 2003. www.sgb.org.br (Resumen).
Kambadur R, Sharma M. Mutations in myostatin (GDF8) in Double-Muscled Belgian Blue and Piedmontese Cattle. Research, Ruakura, Hamilton, New Zealand; 3 US Department of Agriculture (USDA)/Agricultural Research Service (ARS). Meat Animal Research Center, Clay Center. Nebraska 1997; 68933-0166 7:910–915 ©1997. https://doi.org/10.1101/gr.7.9.910 DOI: https://doi.org/10.1101/gr.7.9.910
McPherron AC, Lawer AM, Lee SJ. Double muscling in cattle due to mutations in the myostatine gene. Proc Natl Acad Sci USA 1997; 94: 12457-12461. https://doi.org/10.1073/pnas.94.23.12457 DOI: https://doi.org/10.1073/pnas.94.23.12457
Barbosa JD, Tury E, Pfeifer Barbosa IB, Cunha Dias VR. Hiperplasia muscular congênita (Doppellender, Double Muscling, Culard) em búfalos no Estado do Pará, Brasil. Ciênc Vet Trop 1999; 2: 50-52. https://doi.org/10.1016/j.nmd.2012.11.008
Borges AS, Barbosa JD, Resende LAL, Mota LSLS, Amorim RM, Carvalho TL, Garcia JF, Oliveira-Filho JP, Oliveira CMC, Souza JES, Winand NJ. Clinical and molecular study of a new form of hereditary myotonia in Murrah water buffalo. Neurom Disord 2013; 23: 206-213. DOI: https://doi.org/10.1016/j.nmd.2012.11.008
Borges AS. Estudo clínico, morfológico e molecular da miotonia hereditaria em búfalos da raça Murrah. Tese de Livre Docente em Clínica de Equinos e Ruminantes, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu 2008; p. 94.
Tantia MS, Vijh RK, Mishra B, Bharani KT. Sequence of gdf 8 (myostatin) gene in Bubalus bubalis. Animal Biotechnology 2007; 18: 177-181. https://doi.org/10.1080/10495390601115526 DOI: https://doi.org/10.1080/10495390601115526
Downloads
Published
How to Cite
Issue
Section
License
Policy for Journals/Articles with Open Access
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work
