Light and Electron-Microscopic Studies on the Tubal Tonsil of the Buffalo (Bubalus bubalis)


  • Ibrahim Alhaji Girgiri Department of Veterinary Anatomy, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004, India
  • Pawan Kumar Department of Veterinary Anatomy, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004, India



Buffalo, Follicle-associated epithelium, Lymphoepithelium, M-cells, Tubal tonsil.


The tubal tonsils of 12 adult buffaloes of the local mixed breed were studied using light and electron microscopy. The tonsillar mucosa lined by pseudostratified columnar ciliated epithelium with goblet cells was modified into lymphoepithelial, due to its association with underlying lymphoid tissue. The lymphoepithelial further modified into follicle-associated epithelium (FAE) characterised by absence of the ciliated cells, goblet cells and the presence of more lymphocytes. The FAE exhibited varying modifications and presented M-cells intimately associated with lymphocytes. At places, the change of the epithelium also showed the presence of specialised M-cell like cells without any association with lymphoid tissue. The lymphoid tissue was in the form of isolated lymphocytes, diffuse aggregations and follicles. The goblet cells of the respiratory epithelium and the glandular acinar cells showed positive activity for the different carbohydrate moieties like acidic and neutral mucopolysaccharides, glycogen, mucins, weakly sulfated acidic mucosubstances, hyaluronic acid and sialomucins. Scanning electron microscopy of the mucosal surface presented a dense mat of cilia, and the FAE exhibited a heterogeneous population of microvillus and M-cells. Transmission electron-microscopy demonstrated the different cell organelles of the various epithelia as well as the cellular profiles of the propria-submucosa, including the high endothelial venules where lymphocytes migration by both inter-endothelial and transvascular routes was also observed. The structural features of the tubal tonsil suggest that new strategies are required to explore this tonsil for targeted delivery of drugs and develop more effective vaccines by the intranasal route.


Bernstein JM, Yamanaka N, Nadal D. Immunobiology of the tonsils and adenoids In: Handbook of mucosal immunology, Ogra PL, Mestecky J, Lamm ME, Strober W, McGhee JR, Bienenstock J. (eds). Academic Press, San Diego 1994. DOI:

Brandtzaeg P. Immunobiology of the tonsils and adenoids. Mucosal Immunol 2015; 2: 1985-2016. DOI:

Chacker A. Anatomy and microanatomy of tonsils. Ency Immunobiol 2015; 3: 420-26. DOI:

Pabst R. Plasticity and heterogeneity of lymphoid organs. What are the criteria to call a lymphoid organ primary, secondary or tertiary? Immunol Lett 2007; 112: 1-8. DOI:

Cesta MF. Normal structure, function and histology of mucosa-associated lymphoid tissue. Toxicol Pathol 2006; 34: 599-608. DOI:

Bockman DE, Cooper MD. Pinocytosis by epithelium associated with lymphoid follicles in the bursa of Fabricius, appendix, and Peyer’s patches. An electron microscopic study. Am J Anat 1973; 136: 455-78. DOI:

Kumar P, Timoney JF, Sheoran AS. M cells and associated lymphoid tissue of the equine nasopharyngeal tonsil. Equine Vet J 2001; 33: 224-30. DOI:

Neutra MR, Mantis NJ, Kraehenbuhl JP. Collaboration of epithelial cells with organised mucosal lymphoid tissues. Nat Immunol 2001; 2: 1004-09. DOI:

Perry M, Whyte A. Immunology of the tonsils. Immunol Today 1998; 19: 414-21. DOI:

Kumar P, Timoney JF. Immunohistochemistry and ultrastructure of the equine tubal tonsil. Anat Histol Embryol 2005; 34: 141-48. DOI:

Ranjit, Kumar P, Kumar P, Singh G. Histology, histochemistry and scanning electron microscopy of tubal tonsil of the young pigs. Vet Res Int 2015; 3: 1-6.

Girgiri IA, Kumar P. Histology, histochemistry and ultrastructure of the nasopharyngeal tonsil of the buffalo (Bubalus bubalis). Anat Histol Embryol 2019; 48: 375-83. DOI:

Girgiri IA, Kumar P. Histological and histochemical studies on the lingual tonsil of the buffalo (Bubalus bubalis). J Buff Sci 2019; 8: 68-76. DOI:

Luna LG. Manual of histologic staining methods of Armed Forces Institute of Pathology. 3rd ed. New York: McGraw-Hill Book Company 1968.

Crossman GA. A modification of Mallory's connective tissue stain with a discussion of principles involved. Anat Rec 1937; 69: 33-8. DOI:

Pearse AGE. Histochemistry: theoretical and applied. 3rd ed. London: Churchill Livingstone 1968.

Cocquyt G, Baten T, Simoens P, Van den Broeck W. Anatomical localisation and histology of ovine tonsils. Vet Immunol Immunopathol 2005; 107: 79-86. DOI:

Kumar P, Kumar P. Histology, histochemistry and scanning electron microscopic studies on the tubal tonsil of the sheep. Indian J Anim Sci 2012; 82: 61-63. DOI:

Casteleyn C, Breugelmans S, Simoens P, Van den Broeck W. The tonsil revisited: Review of the anatomical localisation and histological characteristics of the tonsils of domestic and laboratory animals. Clin Dev Immunol 2011. DOI:

Indu VR, Lucy KM, Chungath JJ, Ashok N, Maya S. Histology and scanning electron microscopy of the tubal tonsil of goats. Vet World 2015; 8: 1011-14. DOI:

Liu Z, Yu Q, Li P, Yang Q. Histological and ultrastructural examinations of porcine tonsils. Anat Rec 2012; 295: 686-90. DOI:

Yang C, Yuan G, Xu Z, Shoa B, Wang J. The topography and the microscopic structure of tonsils in the adult Bactrian camel (Camelus bactrianus). J Camel Pract Res 2011; 18(2): 155-63.

Corr SC, Cormac CGM, Gahan Colin H. M-cells: origin, morphology and role in mucosal immunity and microbial pathogenesis. FEMS Immunol Med Microbiol 2008; 52: 2-12. DOI:

Hathaway LJ, Kraehenbuhl JP. The role of M-cells in mucosal immunity. Cell Mol Life Sci 2000; 57: 323-32. DOI:

Jang MH, Kweon MN, Iwatani K, Ya,mamoto M, Terahara, K, Sasakawa C, Suzuki T, Nochi Tyokota Y, Rennert PD, Hiroi T, Tamagawa H, Iijima H, Kunisawa J, Yuki Y, Kiyono H. Intestinal villous M cells: an antigen entry site in the mucosal epithelium. Proc Natl Acad Sci 2004; 101: 6110-15. DOI:

Bye WA, Allan CH, Trier JS. Structure, distribution, and origin of M cells in Peyer’s patches of mouse ileum. Gastroenterol 1984; 86: 789-801.

Pappo J. Generation and characterisation of monoclonal antibodies recognising follicle epithelial M cells in rabbit gut-associated lymphoid tissues. Cell Immunol 1989; 120: 31-41. DOI:

Gebert A, Hach G, Bartels H. Co-localization of vimentin and cytokeratins in M-cells of rabbit gut-associated lymphoid tissue (GALT). Cell Tissue Res 1992; 269: 331-40. DOI:

Knoop KA, Kumar N, Butler BR, Sakthivel SK, Taylor RT, Nochi T, Akiba H, Yagita H, Kiyono H, Williams I. RANKL is necessary and sufficient to initiate development of antigen-sampling M cells in the intestinal epithelium. J Immunol 2009; 183: 5738-47. DOI:

Savidge TC. The life and times of an intestinal M cell. Trends Microbiol 1996; 4: 301-6. DOI:

Casteleyn C, Cornelissen M, Simoens P, Van den Broeck W. Ultramicroscopic examination of the ovine tonsillar epithelia. Anat Rec 2010; 293: 879-89. DOI:

Allan CH, Mendrick DL, Trier JS. Rat intestinal M cells contain acidic endosomal-lysosomal compartments and express class II major histocompatibility complex determinants. Gastroenterol 1993; 104: 698-708. DOI:

Indrasingh I, Chandi G, Vettivel S. Route of lymphocyte migration through the high endothelial venule (HEV) in human palatine tonsil. Annals Anat 2002; 184: 77-84. DOI:

Hafeez A, Khan MY, Minhas LA. The relative distribution of high endothelial venules in the subepithelial lymphoid compartments of human palatine tonsil. Annals Pak Inst Med Sci 2008; 4: 223-26.

Zidan M, Pabst R. The microanatomy of the palatine tonsils of the one-humped camel (Camelus dromedarius). Anat Rec 2009; 292: 1129-97. DOI:

Hayashi S, Kikuta A, Ohtsuka A, Masuda Y. Microvascular architecture of rat nasal associated lymphoid tissue. Arch Histol Cytol 1991; 5: 279-87. DOI:

Zidan M, Jecker P, Pabst R. Differences in lymphocyte subsets in the wall of high endothelial venules and the lymphatics of human palatine tonsils. Scand J Immunol 2000; 51: 372-76. DOI:

Marchesi VT, Gowans JL. The migration of lymphocyte through the endothelium of venules in lymph nodes: an electron microscopic study. Proc R Soc Lond 1964; 159: 283-90. DOI:

Stan RV. Structure and function of endothelial caveolae. Microsc Res Tech 2002; 57: 350-64. DOI:

Feng D, Nagy JA, Dvorak HF, Dvorak AM. Ultrastructural studies define soluble macromolecular, particulate, and cellular transendothelial cell pathways in venules, lymphatic vessels, and tumor-associated microvessels in man and animals. Micros Res Tech 2002; 57: 289-326. DOI:

Dvorak AM, Feng D. The vesiculo-vacuolar organelle (VVO): a new endothelial cell permeability organelle. J Histochem Cytochem 2001; 49: 419-31. DOI:

Vasile E, Qu-Hong H, Dvorak F, Dvorak AM. Caveolae and vesiculo-vacuolar organelles in bovine capillary endothelial cells cultured with VPF/VEGF on floating matrigel collagen gels. J Histochem Cytochem 1999; 47: 159-67. DOI:




How to Cite

Girgiri, I. A. ., & Kumar, P. . (2020). Light and Electron-Microscopic Studies on the Tubal Tonsil of the Buffalo (Bubalus bubalis). Journal of Buffalo Science, 9, 60–70.