Novel Isolates of Lactobacilli from Crop of Algerian Poultry as Potential Probiotic for Food Industry
DOI:
https://doi.org/10.6000/1927-3037.2016.05.03.3Keywords:
Lactobacillus, Poultry crop, Probiotic properties, Adhesion.Abstract
This study was aimed at selecting novel strains of Lactobacillus from crop of Algerian poultry. One hundred forty (140) lactobacilli strains were isolated and examined for their potentiality probiotic properties. From these isolated strains, nine appear to possess a probiotic value and highlighted a noticeable heterogeneity. The isolate L. plantarum G1 showed the best inhibitory activity against several indicator strains. Furthermore, the results showed that culture and neutralized supernatants exhibited varying degrees of inhibitory activity against strains of enterobacteria from poultry origin. The tested strains were acid resistant and were also bile tolerant. Antibiotic resistance, co-aggregation activity and hydrophobicity percentage were strain-dependent. Moreover, six strains were able to adhere to epithelial cells. Finally, six Lactobacillus strains, such as strain L. plantarum G1, L. plantarum PC2, L. viridesencs G3, L. helveticus PC6, L. delbrueckii ssp delbrueckii G7 and L. fermentum PC8, showed essential probiotic properties. The identity of the best strain L. plantarum G1 was confirmed by 16S rRNA gene sequence using PCR.
References
Apajalahti J, Kettunen A, Graham H. Characteristics of the gastrointestinal microbial communities, with special reference to the chicken. World Poult Sci 2004; 60(2): 223-32. http://dx.doi.org/10.1079/wps20040017
Garcia-Mazcorro JF, Minamoto Y. Gastrointestinal microorganisms in cats and dogs: a brief review. Arch Med Vet 2013; 45: 111-24. http://dx.doi.org/10.4067/S0301-732X2013000200002
Amit-Romach E, Sklan D, Unil Z. Microflora ecology of the chicken intestine using 16S ribosomal DNA primers. Poult Sci 2004; 83: 1093-8. http://dx.doi.org/10.1093/ps/83.7.1093
Champ M, Szylit O, Raibaud P, Ait-Abdelkader N. Amylase production by three Lactobacillus strains isolated from chicken crop. J Appl Bacteriol 1983; 55: 487- 93. http://dx.doi.org/10.1111/j.1365-2672.1983.tb01689.x
Stanley D, Hughes RJ, Geier MS, Moore RJ. Bacteria within the Gastrointestinal Tract Microbiota Correlated with Improved Growth and Feed Conversion: Challenges Presented for the Identification of Performance Enhancing Probiotic Bacteria. Front Microbiol 2016; 7:187. http://dx.doi.org/10.3389/fmicb.2016.00187
Saxena S, Saxena VK, Tomar S, Sapcota D, Gonmei G. Characterisation of caecum and crop microbiota of Indian indigenous chicken targeting multiple hypervariable regions within 16S rRNA gene. Br Poult Sci 2016; 57: 381-9. http://dx.doi.org/10.1080/00071668.2016.1161728
Muñoz-Atienza E, Gómez-Sala B, Araújo C, Campanero C, Del Campo R, Hernández PE, Herranz C, Cintas LM. Antimicrobial activity, antibiotic susceptibility and virulence factors of lactic acid bacteria of aquatic origin intended for use as probiotics in aquaculture. BMC Microbiol 2013; 13: 15.
http://dx.doi:10.1186/1471-2180-13-15
Chen YJ, Son KS, Min BJ, Cho JH, Kwon OS, Kim IH. Effects of dietary probiotic on growth performance, nutrients digestibility, blood characteristics and faecal noxious gas content in growing pigs. Asian Australas J Anim Sci 2005; 18: 1464-8. http://dx.doi.org/10.5713/ajas.2005.1464
Salminen S, Isolauri E, Salminen E. Clinical uses of probiotics for stabilising the gut mucosal barrier: successful strains and future challenges. Antonie Van Leeuwenhoek 1996; 70: 251-62. http://dx.doi.org/10.1007/bf00395941
Sahnouni F, Matallah-Boutiba A, Chemlal D, Boutiba Z. Technological characterization of lactic acid bacteria isolated from intestinal microbiota of marine fish in the Oran Algeria coast. Afr J Microbiol Res 2012; 6: 3125-33. http://dx.doi.org/10.3923/aj.2012.81.87
Boris S, Jimenez-Diaz R, Caso JL, Barbes C. Partial characterization of a bacteriocin produced by Lactobacillus delbrueckii subsp. lactis UO004, an intestinal isolate with probiotic potential. J Appl Microbiol 2007; 91: 328-33. http://dx.doi.org/10.1046/j.1365-2672.2001.01403.x
Schillinger U, Lucke FK. Antibacterial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol 1989; 55: 1901-6. http://dx.doi.org/10.1111/j.1365-2672.1991.tb02743.x
Lin WH, Yu B, Jang SH, Tsen HY. Different probiotic properties for Lactobacillus fermentum strain isolated from swine and poultry. Anaerobe 2007; 13: 107-13. http://dx.doi.org/10.1016/j.anaerobe.2007.04.006
Yu B, Tsen HY. Lactobacillus cells in the rabbit digestive tract and the factors affecting their distribution. J Appl Bacteriol 1993; 75: 269-75. http://dx.doi.org/10.1111/j.1365-2672.1993.tb02776.x
Rosenberg M, Gutnick D, Rosenberg E. Adherence of bac-teria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Lett 1980; 9: 29-33. http://dx.doi.org/10.1111/j.1574-6968.1980.tb05599.x
Beaur AW, Kirbym WMM, Sherris JC, Turck M. Antibiotics susceptibility testing by a standardized single disc method. Am J Clin Pathol 1966; 45: 493- 6.
Barnes EM. The intestinal microflora of poultry and game birds during life and after storage. J Appl Bacteriol 1979; 46: 407-19. http://dx.doi.org/10.1111/j.1365-2672.1979.tb00838.x
Fuller R. The importance of lactobacilli in maintaining normal microbial balance in the crop. Br Poult Sci 1977; 18: 85-94 http://dx.doi.org/10.1080/00071667708416332
Barrow PA, Brooker BE, Fuller R, Newport MJ. The attachment of bacteria to the gastric epithelium of the pig and its importance in the microecology of the intestine. J Appl Bacteriol 1980; 48: 147-54. http://dx.doi.org/10.1111/j.1365-2672.1980.tb05216.x
Garriga M, Pascual M, Monfort JM, Hugas M. Selection of lactobacilli for chicken probiotic adjuncts. J Appl Bacteriol 1998; 84: 125-32. http://dx.doi.org/10.1046/j.1365-2672.1997.00329.x
Fons M, Hege T, Ladire M, Raibaud P, Ducluzeau R, Maguin E. Isolation and caracterization of a plasmid from Lactobacillus fermentum conferring erythromycin resistance. Plasmid 1997; 37: 199-203. http://dx.doi.org/10.1006/plas.1997.1290
Daeschel MA. Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food Technol 1989; 43: 164-7.
Xanthopoulous V, Litopoulou-Tzanetaki E, Tzanetaki N. Characterization of Lactobacillus isolates from infant faeces as dietary adjuncts. Food Microbiol 2000; 17: 205-15. http://dx.doi.org/10.1006/fmic.1999.0300
Conway PL, Gorbach SL, Goldin BR. Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J Dairy Sci 1987; 70: 1-12. http://dx.doi.org/10.3168/jds.s0022-0302(87)79974-3
Idoui T, Leghouchi E, Karam NE. Selection of Lactobacillus plantarum BJ0021 for rabbit probiotic adjuncts. Int J Probiotics Prebiotics 2007; 2: 188-93.
Gilliland SE, Staley TE, Bush LJ. Importance of bile tolerance of Lactobacillus acidophilus used as dietary adjunct. J Dairy Sci 1984; 67: 3045-51. http://dx.doi.org/10.3168/jds.s0022-0302(84)81670-7
Pisano MB, Viale S, Conti S, Fadda ME, Deplano M, Melis MP, Deiana M, Cosentino S. Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian dairy products. BioMed Res Int 2014; 2014. http://dx.doi:10.1155/2014/286390
Van Tassell ML, Miller MJ. Lactobacillus adhesion to mucus. Nutrients 2011; 3: 613-6. http://dx.doi.org/10.3390/nu3050613
Muñoz-Provencio D, Llopis M, Antolín M, de Torres I, Guarner F, Pérez-Martínez G, Monedero V. Adhesion properties of Lactobacillus casei strains to resected intestinal fragments and components of the extracellular matrix. Arch Microbiol 2008; 191: 153-61. http://dx.doi.org/10.1007/s00203-008-0436-9
Downloads
Published
How to Cite
Issue
Section
License
Policy for Journals/Articles with Open Access
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work
Policy for Journals / Manuscript with Paid Access
Authors who publish with this journal agree to the following terms:
- Publisher retain copyright .
- Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work .
