Studies on Thyroid Hormones and some Biochemical Constituents of Follicular Fluid in Buffalo
DOI:
https://doi.org/10.6000/1927-520X.2014.03.01.5Keywords:
Biochemical constituents, thyroxine (T4), triiodothyronine (T3), follicular fluidAbstract
Present study investigated the levels of some biochemical constituents and thyroid hormones in follicular fluid and serum and compared their concentrations in different size follicles of buffaloes. Buffalo ovaries with unknown reproductive status were collected from abattoir. The follicles visible on its surface were classified based on their diameter as small (<5 mm), medium (5-10 mm) and large (>10 mm) follicles. Twenty four samples from follicles along with blood samples of buffaloes belonging to respective category were collected. The follicular fluid and the serum samples were analyzed for total proteins, albumin, cholesterol, glucose and hormones tri-iodothyronine and thyroxine. The results of the present study revealed that, there was no specific trend observed in the levels of total proteins and albumin according to the size of the follicles but there was significant difference (P<0.01) in the levels of total proteins in follicular fluid and serum. The levels of total cholesterol showed increasing trend as the size of the follicle increases but the variations are not statistically significant. The serum total cholesterol concentration was significantly higher (P< 0.01) than that of follicular fluid. There was increasing trend of glucose concentration in the follicular fluid and also in the serum of respective buffalo with increase in the size of the follicle but the difference was non-significant. The serum glucose concentrations were significantly higher than the levels in the follicular fluid. There was no variation in the serum levels of triiodothyronine between buffalo bearing small and medium sized follicle while, highest serum level was observed in buffalo bearing large sized follicle. The significant (P≤0.01) increase in the levels of T3 in large sized follicle may attribute to the increase in the activity of monodeioniase enzyme. The follicular fluid thyroxine level showed increasing trend with the size of the follicle but the variation was not significant. There was no significant variation in the levels of T4 in serum and follicular fluid in all the groups.
References
Snedecor GW, Cochran WG. Statistical Methods 8th ed. Oxford and IBH Publishing Company, New Delhi 1994.
Shiny J. Follicular fluid protein profile in buffalo 2006. M.V.Sc Thesis submitted to Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, India.
Arshad HM, Ahmad N, Zia-ur-Rahman, Samad HA, Akhtar N, Ali S. Studies on some biochemical constituents of ovarion follicular fluid and peripheral blood in buffaloes. Pakistan Veterinary J 2005; 25(4).
Leroy JL, MR Vanholder T, Delanghe JR, Opsomer G, Van Soom A, Bols PEJ, Dewulf J, Kruif A de. Metabolic changes in follicular fluid of dominant follicle in high yielding dairy cows early post-partum Theriogenology 2004; 62: 1131-43. DOI: https://doi.org/10.1016/j.theriogenology.2003.12.017
Alkalby JMA, Bushra FH, Fahad TA. Study on some hormonal and biochemical constituents of follicular fluid and blood plasma in buffaloes. Bas J Vet Res 2012; 11(1): 13-15. DOI: https://doi.org/10.33762/bvetr.2012.54761
Jindal SK, Chopra SC, Lal D, Singh P, Yadav NK. Studies on some biochemical constituents of the buffalo follicular fluid. Buffalo J 1997; 3: 369-73.
McNatty KP. Follicular Fluid. In R.E. (Ed.) The Vertebrate Ovary: The comparative Biology and Evolution. Plenum Press, New York 1978; pp. 215-259.
Ahmed WM, Shalaby SIA, Zaabal MM. Some biochemical constituents of preovulatory and cystic ovarian follicular fluids in buffalo cows with emphasis on protein polymorphism. Buffalo Bulletin 1997; 16(1): 3-5.
Khan FA, Das GK, Megha P, Mir RA, Uma S. Changes in biochemical composition of follicular fluid during reproductive acyclicity in water buffalo (Bubalus bubalis). Anim Reprod Sci 2011; 127: 38-42. DOI: https://doi.org/10.1016/j.anireprosci.2011.07.013
Abd Ellah MR, Hussein HA, Derar DR. Ovarian follicular fluid constituents in relation to stage of estrus cycle and size of the follicle in buffalo. Veterinary World 2010; 3(6): 263-67.
Thakur RS, Chauhan RA, Singh BK. Studies on biochemical constituents of caprine follicular fluid. Indian Vet J 2003; 80(2): 160-62.
Arosh AJ, Devanathan TG, Kathiresan D, Pattabiraman SR. The Blue Cross Book 8: 6 cited by P. K. Bordoloi et al. Indian Vet J 1997; 77: 2000. 638-39.
Parmar AP, Mehta VMJ. Anim Sci 1991; 61: 1197 as cited by P K Bordoloi et al.. Indian Vet J 2000; 77: 638-639.
Nandi SV, Girish K, Manjunatha BM, Ramesh HS, Gupta PSP. Follicular fluid concentrations of glucose, lactate and pyruvate in buffalo and sheep, and their effects on cultured oocytes, granulosa and cumulus cells. Theriogenology 2008; 69: 186-96. http://dx.doi.org/10.1016/j.theriogenology.2007.08.036 DOI: https://doi.org/10.1016/j.theriogenology.2007.08.036
Abd El-Nasser, Mohammed A. Total protein, urea, glucose, triglycerides and cholesterol concentrations of ruminant follicular fluid in relation to follicle size and estrous stage. Afr J Anim Biomed Sci 2011; 6(1): 123-28.
Ali SN, Ahmad N, Akhtar, Zia-ur-Rahman, Ahmad M. Hormonal profile in the serum and follicular fluid of female camel (Camelus dromedarius) during the peak and the low breeding season. Pakistan Vet J 2011; 31(4): 331-35.
Downloads
Published
How to Cite
Issue
Section
License
Policy for Journals/Articles with Open Access
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are permitted and encouraged to post links to their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work
