Essential Oil Composition, Antimicrobial Potential, Phytochemical Profile and Toxicity of Essential Oils and Crude Extracts of Sweet Basil Prior and After Flowering
DOI:
https://doi.org/10.6000/1927-3037.2012.01.01.06Keywords:
Ocimum tenuiflorum, essential oil, antimicrobial screening, toxicity, medicinal properties, flowering, functional food, phytochemicalsAbstract
Essential oils and crude extracts of sweet basil (Ocimum tenuiflorum - OT) which are mostly used to flavor and spice food were prepared and analyzed prior and after flowering by GC and subjected to in vitro antimicrobial and in vivo toxicity evaluation. The essential oil composition was found to vary considerably with respect to harvesting time (prior to flowering stage- eugenol- 17.7%, carvacrol- 27.1% and methyl-eugenol- 52.1%; as compared to flowering (caryophyllene- 9.26% and methyl-eugenol and comparatively smaller percentages of carvacrol and eugenol). Phytochemical screening of the plant samples from either season showed that the whole plants constituted of alkaloids, flavones, saponins, terpenes, sterols, tannins and triterpenes. The essential oils and crude extracts of plant parts of OT (leaves, twigs and/or inflorescence) in both stages showed marked antimicrobial activity against S. aureus and methicillin-resistant S. aureus and with no apparent toxicity in rats. The observed antimicrobial properties and non-toxic nature during both stages of flowering would tend to validate the medicinal properties of OT as a functional food.
References
Laakso L, Seppanen-Laakso T, Hermann-Wolf B, Knobloch K. Constituents of the essential oil from the Holy basil or Tulsi basil, Ocimum sanctum. Planta Med 1990; 56: 527. http://dx.doi.org/10.1055/s-2006-961084
Maheshwari ML, Singh BM, Gupta R, Chien MJ. Essential oil of sacred basil (Ocimum sanctum). Indian Perfum 1987; 31: 137-45.
Rangasamy O, Raoelison G, Rakotoniriana FE, Cheuk K, Urverg-Ratsimamanga S, Quetin-Leclercq J. Screening for anti-infective properties of several medicinal plants of the Mauritian flora. J Ethnopharmacol 2007; 109: 331-7. http://dx.doi.org/10.1016/j.jep.2006.08.002
Mahomoodally MF, Gurib-Fakim A, Subratty AH. Antimicrobial properties and phytochemical profiles of endemic medicinal plants of Mauritius. Pharm Biol 2005; 43: 1-6. http://dx.doi.org/10.1080/13880200590928825
Kothari SK, Bhattacharya AK, Ramesh S. Essential oil yield and quality of methyl eugenol rich Ocimum tenuiflorum L.f. (syn. O. Sanctum L.) grown in south India as influenced by method of harvest. J Chromatogr A 2004; 1054: 67-72. http://dx.doi.org/10.1016/j.chroma.2004.03.019
Ayoola GA, Lawore FW, Adelowotan T, et al. Chemical analysis and antimicrobial activity of the essential oil of Syzigium aromaticum (clove). Afr J Microbiol Res 2008; 2: 162-6.
Malathi R, Gomaz P. Evaluation of preliminary toxicity studies on the methanolic leave extract of Tylophora asthmatica in experimental rats. J Pharmacol Toxicol 2008; 3: 34-40. http://dx.doi.org/10.3923/jpt.2008.34.40
OECD. Organization for Economic Co-operation and Development. In: Guideline for testing of chemicals: Acute Oral Toxicity-Fixed Dose Procedure 2001; 420.
Ramesh S, Kothari SK, Bhattacharya AK, Garg SN, Khanuja SPS. Volatile constituents in oil from different plant parts of methyl eugenol-rich Ocimum tenuiflorum L.f. (syn. O. sanctum L.) grown in South India. J Essen Oil Res 2005; 17: 656-68.
Laakso L, Seppanen-Laakso T, Hermann-Wolf B, Knobloch K. Constituents of the essential oil from the Holy basil or tulsi basil, Ocimum sanctum. Planta Med 1990; 56: 527. http://dx.doi.org/10.1055/s-2006-961084
Lawrence BM, Hogg JW, Terhune SJ, Pichitakul N. Essential oils and their constituents. IX. The Oils of Ocimum sanctum and Ocimum basilicum from Thailand. Flavor Ind 1972; 47-9.
Aqil F, Khan MSA, Owais M, Ahmad I. Effect of certain bioactive plant extracts on clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus. J Basic Microbiol 2005; 45:106-14. http://dx.doi.org/10.1002/jobm.200410355
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