Localization Analysis of Natural Toxin of Solanum tuberosum L. via Mass Spectrometric Imaging

Authors

  • Riho Hashizaki Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui, 910-1195, Japan
  • Hanaka Komori Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui, 910-1195, Japan
  • Kohei Kazuma Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 930-0194, Japan
  • Katsuhiro Konno Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama 930-0194, Japan
  • Kyuichi Kawabata Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui, 910-1195, Japan
  • Daisaku Kaneko Kyushu Institute of Technology, 1-1 Sensui, Tobata, Kitakyushu, Fukuoka, 804-0015, Japan
  • Hajime Katano Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui, 910-1195, Japan
  • Shu Taira Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui, 910-1195, Japan

DOI:

https://doi.org/10.6000/1927-3037.2016.05.01.1

Keywords:

Food safety, glycoalkaloid, mass spectrometry imaging, a-solanine, a-chaconine.

Abstract

The use of mass spectrometry imaging (MSI) revealed the localization of a-solanine and a-chaconine as natural toxins for Potato (Solanum tuberosum L.). The content of Potato glycoalkaloids, a-solanine and a-chaconine, were quantitatively determined by high performance liquid chromatography (HPLC). Matrix assisted laser desorption/ionization-based tandem mass spectrometry (MS) could determine a-solanine and a-chaconine from raw potato extraction and section. After budbreak, a-solanine and a-chaconine were produced and localized at periderm and germ compared with that before budbreak. At germ region, these glycoalkaloids did not exist whole germ region but eccentrically localize at germ surface and central region. The amount of a-chaconine was twofold higher than a-solanine at periderm. At germ region, there was no difference between these toxins.

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Published

2016-03-01

How to Cite

Hashizaki, R., Komori, H., Kazuma, K., Konno, K., Kawabata, K., Kaneko, D., Katano, H., & Taira, S. (2016). Localization Analysis of Natural Toxin of Solanum tuberosum L. via Mass Spectrometric Imaging. International Journal of Biotechnology for Wellness Industries, 5(1), 1–5. https://doi.org/10.6000/1927-3037.2016.05.01.1

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