Hemostatic Ability of Thermosensitive Biologically Active Gelatin-Alginate Hydrogels Modified with Humic Acids and Impregnated with Aminocaproic Acid

Denis  Miroshnichenko; Katerina  Lebedeva; Vladimir  Lebedev; Аnna  Cherkashina; Sergey  Petrushenko; Ihor  Hrubnyk; Yulia  Yudina; Olena  Bogoyavlenska; Natalja  Klochko; Liudmyla  Lysenko; Mykhailo  Miroshnychenko

doi:10.6000/1929-5995.2024.13.16

Authors

Denis Miroshnichenko The Department of Oil, Gas and Solid Fuel Refining Technologies, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine https://orcid.org/0000-0002-6335-8742
Katerina Lebedeva The Department of Plastics and Biologically Active Polymers Technology, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine
Vladimir Lebedev The Department of Plastics and Biologically Active Polymers Technology, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine https://orcid.org/0000-0001-6934-2349
Аnna Cherkashina The Department of Plastics and Biologically Active Polymers Technology, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine
Sergey Petrushenko Faculty of Physics, V.N. Karazin Kharkiv National University, 4, Svobody Square, 61022, Kharkiv, Ukraine and Technical University of Liberec, 2, Studentska str., 46117, Liberec, Czech Republic Technical University of Liberec, 46117 Liberec, Czech Republic
Ihor Hrubnyk The Common Pharmacy Department, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine
Yulia Yudina The Common Pharmacy Department, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine https://orcid.org/0000-0002-5322-5786
Olena Bogoyavlenska The Department of Oil, Gas and Solid Fuel Refining Technologies, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine
Natalja Klochko Department of Micro- and Nanoelectronics, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine
Liudmyla Lysenko The Department of Oil, Gas and Solid Fuel Refining Technologies, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine
Mykhailo Miroshnychenko The Department of Oil, Gas and Solid Fuel Refining Technologies, National Technical University «Kharkiv Polytechnic Institute», 2, Kyrpychova str., 61002 Kharkiv, Ukraine

DOI:

https://doi.org/10.6000/1929-5995.2024.13.16

Keywords:

Hemostasis, biopolymer hydrogel, gelatin, alginate, humic acid, aminocaproic acid, thermosensivity, blood coagulation

Abstract

The article studies the hemostatic ability of thermosensitive biopolymer hydrogels containing 14% by weight of gelatin and 6.4% by weight of sodium alginate, impregnated with a hemostatic agent aminocaproic acid ~0.2 g/ml, with the addition of humic acids with antioxidant, antibacterial, fungicidal and anti-inflammatory properties. Modification of hydrogels with humic acids slightly increases viscosity, but maintains the gel-sol transition temperature close to the physiological temperature of about 37 °C, which allows them to melt on human skin or inside a wound, ensuring the delivery of aminocaproic acid. SEM images showed that the developed hydrogels have a layered internal morphology, which is improved due to better swelling of the hydrogels contained humic acids, which promotes the dissolution of aminocaproic acid inside the hydrogels and its subsequent rapid delivery to the bleeding site when applying a hydrogel dressing. It has been experimentally established that the concentration of humic acids in hydrogels of no more than 5 wt.% promotes blood clotting due to the entry of aminocaproic acid into it from the hydrogels. The aminocaproic acid delivered at physiological temperature from these hydrogels can shorten the blood clotting time to the lower limit of the normal clotting time range. The clotting time of the hydrogel with 5 wt.% humic acid is only 95 s, which confirms its particularly effective hemostatic ability.

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