Cellular Mechanisms of Saline Extract of Alligator Pepper (Zingiberaceae Aframomum melegueta) for Specific Protection against Fetal Macrosomia
Published: 31 March 2014
Abstract: Introduction: Aqueous extract of Alligator pepper is known to reduce gestational weight gain and litter size in Sprague Dawley rats. This study was done to determine the underlying hormonal and cellular mechanisms of action.
Methods: We performed a controlled cross sectional intervention study with 45 female Sprague Dawley rats, which after acclimatization for two weeks, were allowed to mate with male rats for three days. Thereafter, these female rats were randomly allocated into three groups A, B and C with 15 female rats in each group. These three major groups were further randomly allocated into three subgroups so that each subgroup had 5 female rats. Pregnant female rats in Group A were injected with 2ml of normal saline intraperitoneally on day 4. Pregnant female rats in groups B and C were injected intraperitoneally with 6.7mg/Kg body weight and 13.3mg/ kg body weight of saline extract of Alligator Pepper respectively on day 4. Glucose levels were estimated with glucometer on days 7, 14, and 21. Blood was collected by intra-ventricular puncture and assayed for insulin levels. Observed differences between control and experimental groups were subjected to tests of significance.
Results: Alligator pepper treated pregnant rats had significantly higher serum glucose levels than control group. Low dose and high dose Alligator pepper depressed serum insulin levels in the experimental group on day 7 and days 7 and 14 respectively
Conclusion: Intraperitoneal injection of saline extract of Alligator pepper prevents first and second trimester hyper-insulinemia in pregnant Sprague Dawley rats.Keywords: Cellular Mechanisms, Alligator pepper, Specific Protection, Fetal Macrosomia.
Download Full Article