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Abstract : Comparative Insight of Regulatory Guidelines for Probiotics in USA, India and Malaysia: A Critical Review
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Abstract: Probiotics have always been a unique category of natural products due to established evidences of their applications in wellness of human beings. Inspite of being based on live microorganisms, commercial exploration of probiotics as biologics, pharmaceuticals, food and nutritional supplements has witnessed a tremendous increase due to their potential of providing health benefits. Currently different regulatory bodies across the globe consider probiotics under several categories depending upon their intended use. In order to clear the ambiguity related to regulatory specifications, assurance of quality and premarketing safety assessment for drafting of comprehensive guidelines with global acceptance is need of the hour. The aim of this paper is to compare existing regulations in countries like United States, India and Malaysia to develop harmonized guidelines for approval of probiotics. Keywords: Dietary supplements, Functional food, Lactobacillus, Probiotics, Regulatory guidelines, FDA.Download Full Article |
Abstract : Co-Production of Cellulase and Xylanase Enzymes By Thermophilic Bacillus subtilis 276NS
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Abstract: Co-production of thermostable extracellular cellulase and xylanase was investigated using bacterial soil isolate. To evaluate the effect of culture conditions on the c-oproduction of both enzymes by Bacillus subtilis 276NS (GenBank accession number JF801740), a Plackett-Burman fractional factorial design was applied. Among the tested variables, yeast extract, sucrose and incubation time were the most significant variables increased cellulase and xylanase productions. Both of xylan and CMC (Carboxymethyl Cellulose) induced the xylanse enzyme production. A near-optimum medium formulation was obtained which increased the cellulase and xylanase enzymes 5.7- and 1.08 –fold higher than the yield obtained with the basal medium, respectively. Thereafter, the response surface methodology was adopted to acquire the best process conditions among the selected variables (xylan, CMC and Yeast Extract (YE)) required for improving xylanase yield. The optimal combinations of the major medium constituents for xylanase production evaluated using non-linear optimization algorithm of EXCEL-solver, was as follows (g/L): D-sucrose, 10; xylan, 10.367, CMC, 10.535; (NH4)2SO4, 1.0; YE, 1.71; Tween-80, 0.4 and FeSO4, 0.25 mg/L, at pH 8.0, temperature 35◦C and incubation time 24h under shaking. The predicted optimum thermostable xylanase activity was 360 U/ml, which was around 4-times the activity with the basal medium. Keywords: Thermostable enzymes, Experimental design, Bacillus, Medium Optimization, Enzyme production.Download Full Article |
Abstract : Changing the Economics of Organic Waste Disposal Using Managed Ecosystem Fermentation
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Abstract: Concentrated organic waste is a major societal problem. It is a disease vector, a source of groundwater contamination, as well as a source of greenhouse gases. Managed Ecosystem Fermentation (MEF) is a technology that converts this societal problem into an economic resource for the community. MEF is a fermentation process that uses over 3,000 species of microbes simultaneously to produce multiple high-value products used in industry and agriculture. The products include fertilizer, high-protein animal feed, volatile fatty acids, longer chain fatty acids, amino acids, enzymes, etc. The values of these products range from $50 to over $16,000 per ton. MEF is an adaptive system that processes non-homogeneous, non-sterile organic waste/s under non-sterile conditions. It converts the waste into industrial products in 24 hours using a microbial system that has worked for millions of years. It is the only known technology that can convert cellulose into protein. Society benefits from converting what is now a cause of disease, groundwater contamination and greenhouse gases into valuable products. Keywords: Managed Ecosystem Fermentation, enzyme, economic resource, MEF, rumen.Download Full Article |
Abstract : Sequencing of 28SrRNA Gene for Identification of Trichoderma longibrachiatum 28CP/7444 Species in Soil Sample
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Abstract: Most of the Trichoderma species are morphologically very similar and were considered for many years as a single species. Since new species were discovered, a consolidated taxonomical scheme was needed and proposed and defined nine morphological species aggregates. DNA methods brought additional valuable criteria to the taxonomy of Trichoderma which are being used today for studies that include identification and phylogenetic classification. Most isolates of the genus Trichoderma that were found to act as mycoparasites of many economically important aerial and soil-borne plant pathogens. Trichoderma has attained importance for substitute of chemical pesticides and hence an attempt was intended to corroborate the positive relatedness of molecular and morphological characters. A fungal strain of Trichoderma longibrachiatum 28CP/7444 was isolated from a soil sample collected from Barabanki district of Uttar Pradesh, India. The universal primers were used for amplification of the 28S rRNA gene fragment and strain characterized by using 28S rRNA gene sequence with the help of ITS marker. It is proposed that the identified strain Trichoderma longibrachiatum 28CP be assigned as the type strain of a species of the genus Trichoderma based on phylogenetic tree analysis together with the 28S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases. The sequence was deposited in GenBank with the accession number JX978541. Thus an integrated approach of morphological and molecular markers can be employed to identify a superior strain of Trichoderma for its commercial exploitation. Keywords: 28S ribosomal RNA gene, Trichoderma, phylogenetic analysis, ITS, DNA sequencing, GenBank.Download Full Article |
