Ultrasonographic Biometrical Studies on Reproductive Organs of rbST Treated Pregnant Kundhi Buffaloes
Pages 52-58
A.B. Kachiwal, B.A. Sheikh, S.A. Sheikh, K.H. Memon, S. Memon and T.A. Qureshi

DOI: http://dx.doi.org/10.6000/1927-520X.2015.04.02.4

Published: 07 August 2015

Abstract: Biometrical studies were conducted on sixty four gravid uteri with estimated gestation period of 1st, 2nd, 3rd and 4th month of pregnancy in Kundhi buffaloes. Sixteen specimens for each month of gestation were collected from Hyderabad slaughterhouse to evaluate techniques for measurement of reproductive organs. The measurements for fetometry and morphometry of reproductive organs by ultrasound machine and manually (by measuring tape and vernier caliper) were performed and compared. The Ultrasound examinations were performed through diagnostic ultrasound machine equipped with multi-frequency 5-10MHz transrectal linear array transducer. Analysis revealed that the ovary, foetus, foetal fluid and uterus progressively increased significantly (P<0.01) in weight during 4th month of the gestation than 1st, 2nd and 3rd month of gestation period in rbST treated Kundhi buffaloes. Ovaries with CL were significantly (P<0.01) heavier than the ovary in which CL was absent. Corpus leutum was significantly lighter (P<0.01) during 1st month of pregnancy than 2nd, 3rd and 4th month of pregnancy in rbST treated Kundhi buffaloes. Gravid uterus was significantly heavier (P<0.01) during 4th month of pregnancy than 1st, 2nd and 3rd month of pregnancy in rbST treated Kundhi buffaloes. There was no significant difference between the measurements of oviduct, cervix and vagina during 1st to 4th month of pregnancy. The placenta, foetal fluid and foetus progressively increased significantly in size as gestation period progressed. The ultrasonographic fetometric and morphometric threshold of CRL, TD and UD and foetal weight was steadily increased in rbST treated Kundhi buffaloes. There was significant increase (P<0.05) in the CRL, TD, UD and foetus weight/volume at 4^th month of pregnancy than 1^st , 2^nd and 3^rd month of pregnancy in rbST treated Kundhi buffaloes. In conclusion, the overall data indicated that the examination for ultrasonographic fetometry and morphometry of reproductive organs of kundhi buffaloes for evaluation of fetal development, estimation of gestational age and formulation of fetal chart through reproductive organs were more suitable as compare to manual biometrical evaluation.

Usage of Saliva as Alternative Biological Fluid to Serum for Minerals, Energetic and Hormones Assessment in Lactating Egyptian Water Buffaloes
Pages 108-113
Abdelghany Hefnawy, Saad Shousha, Omnia Abdelhamid and Seham Youssef

DOI: http://dx.doi.org/10.6000/1927-520X.2013.02.03.1

Published: 30 November 2013

Abstract: Blood sample is the most common biological fluid utilized for diagnosis and monitoring of diseases. Saliva contains locally produced substances as well as serum component, so the aim of this study is to compare the profile of minerals, energetic and hormones in Egyptian water buffaloes. Blood serum and saliva samples were collected from 80 healthy multiparous, non- pregnant lactating Egyptian water buffaloes. Both fluids were tested for sodium, potassium, chloride, calcium, phosphorous, magnesium, insulin, cortisol, ACTH, glucose, urea, creatinine, total protein and immunoglobulin [IgA]. The results revealed that, serum concentrations of calcium, glucose, total protein, sodium, chloride, Insulin, cortisol, ACTH and IgA were significantly higher than saliva. In contrast, the concentrations of potassium and phosphorous in the saliva were significantly higher than that of serum. On the other hand no significant change in respect of urea, creatinine and magnesium was noted between saliva and serum. The relationships between saliva and serum of the estimated parameters were significantly positive except the concentrations of insulin in saliva and blood serum did not correlate. In conclusion, the saliva sample can be used in clinical practice with high level of reliability and provide non-invasive biological fluid for monitoring of different parameters in Egyptian water buffaloes.

Ultrasonographic Evaluation of Teat Structure and Detection of Prominent Annular Folds in Brazilian Dairy Buffaloes- Pages 55-61

Lilli Bittner, Rudiger Daniel Ollhoff, José Diomedes Barbosa Neto, Joachim Spilke, Fabio Celidonio Pogliani, José Lino Martinez and Alexander Starke

DOI: https://doi.org/10.6000/1927-520X.2019.08.03.1

Published: 09 November 2019

Abstract: Udder and teat characteristics differ between buffaloes (Bubalus bubalis) and cattle (Bos taurus) and the differences are important in relation machine milk ability. Dairy buffalo milk out more slowly and are more difficult to milk than dairy cows, especially when milking machines are used. The goal of this study was to determine the ultrasonographic characteristics of udder and teat morphology in dairy buffaloes in Brazil, a country with a relatively recent history of buffalo farming. External assessment of the udder type and ultrasonography of the teat were done in 63 purebred Murrah and Mediterranian  Murrah dairy buffalo cows on three farms with different management systems. Udder types differed among farms; the rear-heavy udder type was most prevalent on two farms and the ball-shaped type occurred frequently on the third farm. Ultrasonographic measurements of the teat were made on one farm and showed correlations between the width of the teat tip and teat canal length, between the width of the teat tip and teat wall thickness and between the width of the teat tip and teat length. During the ultrasonographic examination of the teat, hyperechogenic membrane-like structures in the region of the annular folds, referred to as prominent annular folds (pAFs), were detected in at least one teat in 98 % of all buffaloes. These folds caused narrowing of the lumen between the teat cistern and gland cistern, but their physiological function and relevance concerning machine milk ability remain unclear. Further studies are needed to evaluate the effect of prominent annular folds on milk flow characteristics during mechanical milking in dairy buffaloes.

Keywords: Dairy buffalo, udder, teat structure, prominent annular folds, ultrasound.

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