jpans
Abstract : Methylsulfonylmethane (MSM) as a New Target for the Treatment of Glycerol-Induced Nephropathy
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Abstract: Objective: Methylsulfonylmethane (MSM) is a naturally occurring nutrient composed of sulfur, oxygen and methyl groups. MSM may have anti-inflammatory and free radical scavenging activity. The present work was done to investigate the possible cure effect of methylsulfonylmethane on glycerol-induced acute renal failure (ARF) in rats. Methodes: After water deprivation, a kidney injury was induced in rats by intramuscular administration of glycerol 10 mL/kg (50% vol./vol. in saline). Several parameters including macroscopic score, histopathological and biochemical were determined to assess the degree of treatment. Results: Results showed that MSM decreased macroscopic and microscopic kidney’s injury scores caused by glycerol. MSM also significantly reduced urea and creatinine levels compared to glycerol-induced ARF group. Conclusion: MSM as a natural product has a curing effect against glycerol-induced myoglobinuria. Keywords: Methylsulfonylmethane (MSM), Acute renal failure, Myoglobinuria. |
Abstract : The Effect of Moringa-Based Supplementation on Fetal Birth Weight in Jeneponto Regency
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Abstract: Background: Low birth weight (LBW) is one of the main causes of morbidity and mortality besides preterm birth. Proper interventions during pregnancy can prevent an adverse pregnancy outcome. This study aims to see the effect of Moringa leaf capsule on birth weight. Methods: This study was double blind randomized controlled trial (DB-RCT) which consisted of three groups namely, Moringa powder (PG), Moringa extract (EG), and iron-folate (IG) groups. The intervention was given for 12 weeks. The samples were 453 pregnant women in six sub-districts in Jeneponto Regency. Data on birth weight and placental weight were measured by trained midwives. The weight of the placenta was measured to determine the placental ratio to birth weight. In addition, some socio-economic variables such as age, gestational age, eating frequency, smoking, and Hb levels were measured in this study. Logistic and linear regression were conducted in this study. Result: The Moringa leaf supplementation groups (PG and EG) delivered child with better weight than iron supplementation (3240.03±453.82, 3161.91±527.70, 3100.89±412.15, respectively). The placenta to birth weight ratio (PBWR) showed that IG group became lowest, merely 16.19%. The most influenced factor to LBW is the unhappiness of the women with her pregnancy (OR = 26.3, 95% CI = 1.227 - 566.474, p = 0.037). Conclusion: Moringa powder supplementation can be used as an alternative in improving new born baby weight. Pregnant women need to be happy and avoid stress to prevent LBW. Keywords: Low birth weight, iron supplementation, maternal nutritional status, Moringa Oleifera. |
Abstract: Inspection of Phytochemical Content and In Vitro Antioxidant Profile of Gnaphalium luteoalbum L.: An Unexplored Phytomedicine
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Abstract: Background: Oxidative stress is intensely linked with several pathological manifestations. Searching for medicinal plant with the superior safety profile for the treatment of oxidative stress related disorders are ongoing due to multiple unwanted effects associated with synthetic antioxidants. Therefore the purpose of this study was to examine the phytochemical content, in vitro antioxidant potentiality of crude methanol extract (CME), carbon tetrachloride fraction (CTF), petroleum ether fraction (PEF), chloroform fraction (CLF) and ethyl acetate fraction (EAF) of aerial parts of Gnaphalium luteoalbum (GL) L. Methods: The aerial parts of the GL were extracted with methanol followed by fractionation using carbon tetrachloride, petroleum ether, chloroform and ethyl acetate. The phytochemical screening of this plant was performed by using standard methods to evaluate the existence of alkaloids, carbohydrates, phenols, flavonoids, saponins, tannins, terpenoids and fixed oils. Antioxidant potentiality was estimated by, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH) and nitric oxide (NO) radical scavenging tests. Total antioxidant capacity (TAC), total phenolic content (TPC) and total flavonoid content (TFC) were also measured. Results: Phytochemical analysis of the aerial parts of GL confirmed the presence of carbohydrates, phenols, flavonoids and saponins in crude extract and its all fractions. The CME showed the highest scavenging activity (43.28%) with IC50 of 398.49 μg/mL in the DPPH radical scavenging test. The IC50 values of EAF, CME were statistically significant (P < 0.05, P < 0.01) with respect to ascorbic acid (ACA). For OH and NO radical scavenging tests maximum scavenging (48.39%, 69.64%) was also reported for CME compared to CTF, PEF, CLF and EAF. Compared to ACA, in case of OH and NO radical scavenging activities the IC50 values of CME were markedly significant (P < 0.01, P < 0.05). In the TAC test, CME showed the highest antioxidant activity (absorbance, 2.6 nm) related to other fractions. TPC was found to be the highest in the CME (115.96 mg of gallic acid equivalent/g of dried extract) rather than other fractions. The ranking order of CTF, PEF, CLF, EAF and CME for TFC was 48.67 < 55.75 < 65.29 < 71.35 < 82.29 mg quercetin equivalent/g of dried extract. Conclusion: The existing study suggested that CME of the aerial parts of GL can be used as a natural source of antioxidant which might be effective towards preventing or slowing oxidative stress related disorders. Keywords: |
Abstract : Effects of Antrodia Camphorata Mycelia Extract Containing Antroquinonol on Lowering Low-Density Lipoprotein Cholesterol: A Randomized Double-Blind Study
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Abstract: Objective: Antrodia camphorata is a type of true fungus that grows only on Cinnamomum camphora trees, also known as Cinnamomum kanehirae (“kashi”) in Taiwan. Antroquinonol is a characteristic component of A. camphorata mycelia extract and was previously shown to exhibit antitumor action and lower blood cholesterol (total cholesterol and low-density lipoprotein [LDL] cholesterol) in cellular and animal models. So, This study examined the ability of A. camphorata mycelia extract to reduce LDL cholesterol in humans. Methods: We conducted a randomized double-blind trial in 26 subjects with either borderline LDL cholesterol (120–139 mg/dL; n = 11) or mildly elevated LDL cholesterol (140–159 mg/dL; n = 15). Participants ingested tablets containing either 25 mg of A. camphorata mycelia extract (antroquinonol: 0.68 mg; n = 14) or a placebo (n = 12) for 12 weeks. Results: The test group showed a significant reduction in LDL cholesterol when compared with the placebo group after 12 weeks of tablet ingestion (p < 0.05), demonstrating the effects of A. camphorata mycelia extract on LDL cholesterol. A. camphorata mycelia extract also tended to reduce total cholesterol when compared with the placebo (p < 0.10). The borderline LDL cholesterol and mildly elevated LDL cholesterol subgroups showed a significant reduction in LDL cholesterol in subjects who ingested A. camphorata mycelia extract compared with those who ingested the placebo, again demonstrating the LDL cholesterol-lowering effect of the extract. Conclusion: A. camphorata mycelia extract lowers LDL cholesterol in individuals with somewhat high LDL cholesterol levels. This clinical trial was registered with the University Hospital Medical Information Network (UMIN no. # 000019670). Keywords: |
