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Abstract : NACA (Nascent-Polypeptide-Associated Complex α Subunit) Against Apoptosis in B Lymphoma Cell is Independent of β Subunit (NACB)
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Abstract: We found depletion of NACA in two kinds of B lymphoma cell lines, Raji and Kapas, were able to induce apoptosis in this study. We also explored whether depletion of β subunit had the same effect, and we were interested in which domain of NACA was potentially responsible to this anti-apoptosis function. Lentivirus-based shRNA was used to deplete endogenous NACA or NACB. Those cells’ viabilities were measured by Alamar-blueTM assay. Cell apoptosis was identified by molecular markers caspase9 and PARP, as well as cellular markers Annexin V and propidium iodide (PI) staining. NACA mutants were constructed by PCR site-directed mutagenesis and delivered into cells by Lentivirus. Immunofluoresce was used to investigate cellular distribution in 293FT cells. Our results demonstrated that the depletion of NACA, but not NACB, was able to induce apoptosis. Deletion of middle or C-term rather than N-term induced obvious apoptosis. The middle part of NACA was response to bind NACB and form a complex. Without middle part, NACA redistributed into nuclei. We conclude NACA against apoptosis is independent of β subunit. C-term of NACA, which is identified as ubiquitin binding domain, and may take important role in anti-apoptosis function. Keywords: NACA, B-cell lymphoma, anti-apoptosis, depletion mutant.Download Full Article |
Abstract : Case Report: Coexistence of Non-Keratinizing Squamous Cell Carcinoma and Follicular Lymphoma in Nasopharynx
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Abstract: We report a very rare case of coexistence of non-keratinizing nasopharyngeal carcinoma and follicular lymphoma in nasopharynx. A 52-year-old woman was admitted in our hospital because of painless enlarged bilateral cervical mass. Nasopharyngoscopy revealed a nasopharyngeal mass, and biopsy showed follicular lymphoma cells infiltrating non-keratinizing squamous carcinoma. The patient underwent combined treatment which targeted two tumors and was alive without any progression in one-year follow up. Keywords: Nasopharyngeal carcinoma, follicular lymphoma.Download Full Article |
Abstract : Vanadium: Possible Use in Cancer Chemoprevention and Therapy
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Abstract: Vanadium belongs among the microelements and plays a role in human nutrition. However, it is not regarded as an essential micronutrient. Vanadium affects various biochemical processes and when present in the body, it is capable of interacting with a notable number of enzymes e.g. protein kinases, phosphatases, ATPases, peroxidases, ribonucleases, oxidoreductases and others. It is documented in scientific literature that vanadium takes part in biochemical processes in mammals. Vanadium is not carcinogenic but its presence in cancer cells and its interactions with many key enzymatic processes results in modified expression of p53 and Bax and in down regulation of Bcl2 proteins and in antiproliferative activity. Anti-carcinogenic and anticancer effects of vanadium in various forms have been demonstrated using in vitro and in vivo experiments. Presently, epidemiologic and clinical studies are necessary for developing a clinically useful, vanadium-based anticancer agent/drug for chemoprevention of cancer. This review summarizes recent scientific information on the role and potential use of vanadium in cancer chemoprevention and cancer therapy. Keywords: Vanadium, vanadium-containing compounds, anti-cancer activity, chemoprevention, anti-carcinogenic effect, apoptosis, antiproliferative activity.Download Full Article |
Abstract : Anticancer Activity of Five Traditionally Used Medicinal Plants’ Extracts
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Abstract: Natural products play a critical role in cancer prevention and therapy today. There are numbers of anticancer agents from natural products used in the clinic.Fighting cancers with novel natural products, especially those extracted from plants, is a potential strategy to develop new anticancer drugs..In the following study, various extracts of well known medicinal plants named Holoptelea integrifolia (F), Operculina turpethum (R), Cardiospermem halicacabum L (S), Dilonix regia (F), Sesbania grandifora seed have been studied for evaluating their anticancer activity. Our data showed that the cytotoxic activity of Operculina turpethum (R) ethanolic extractwas relative high for all 6 cancer cell lines as compared to other extracts. The active compound and anticancer mechanism of these extracts are worth investigating in the future. Keywords: Operculina turpethum, EtOH extract, Cell line cytotoxic activity, MTT bioassay.Download Full Article |
