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ijbwi

FluDen Primer DB –PCR Primer Database for Influenza A and Dengue Virus
Pages 92-96
Raghavendra Krishnappa and Rajendran Paramasivam

DOI: http://dx.doi.org//10.6000/1927-3037.2015.04.03.2

Published: 01 December 2015

Open Access 


Abstract: FluDen Primer Database (http://www.fludenpdb.com) has been designed and developed as a web application program to provide free access to the in-silico designed multiple potential primers for PCR detection and quantification assays for Influenza and dengue viruses. This program also permits user to submit sequence of their choice for primer design. The database contains primer records for Influenza and dengue viruses which cause infection in Humans. As of 2014 there are 142 primer sets for screening 32 genes/regions of Influenza and dengue viruses together. Application contain gene information, assay details such as oligonucleotide sequence, primer properties and reaction conditions, publication information. We have developed a resource, FluDen Primer DB which contains primer that can be used for PCR under provided amplification conditions for each primer pair. A distinguish feature of the FlueDen is the primers listed in DB are the products of PCR design application which are experimentally validated. Primers for the FluDen were designed using current genomic information available from the National Center for Biotechnology Information (NCBI).

Keywords: PCR, Influenza, Dengue, NetPrimer, FastPCR.
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ijbwi

Optimization Processing Parameters for Curcuma xanthorriza Oleoresin Yield and its Antioxidant Activity
Pages 97-102
Zarani M. Taher and Mohamad R. Sarmidi

DOI: http://dx.doi.org//10.6000/1927-3037.2015.04.03.3

Published: 01 December 2015

Open Access 


Abstract: Processing is a critical aspect of herbal based products, and processing method is known to affect the content, activity and bioavailability of bioactive compounds. Extraction of oleoresin from Curcuma xanthorriza is effected by several processing parameters. Appropriate solvent selection such as polar solvent result in better extraction yield while lower polarity solvent end up with extracts having higher concentration of active compounds. In this study, different processing parameters were carried out including blanching treatment (boiled, steamed) at range of time (24 hours, 48 hours and 72 hours), solvent of extraction (methanol, ethanol and acetone). The curcuminoid content and antioxidant activity were determined in Curcuma xanthorriza oleoresin. The result obtained was proved that ethanol was the most effective solvent. Blanching treatment affects the yield of oleoresin and promotes the release of curcuminoids content.

Keywords: Curcuma xanthorriza, optimisation, blanching, solvent, antioxidant activity.
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International Journal of Biotechnology for Wellness Industries

Research Progress on the Injury Mechanism and the Protective Effect of Blood-Brain Barrier
Pages 51-56
Han-Wen Yan, Fang-Yan He, Wei-Li Wang, Li-Song Liu, Qing Lin and Xiao-Hua Duan

DOI: http://dx.doi.org/10.6000/1927-3037.2015.04.02.1

Published: 28 July 2015

Open Access 


Abstract: The Blood-Brain Barrier (BBB) is important structure to maintaining the stabilization of central nervous system. It is composed of endothelial cell and tight junction, the basal lamina, astrocytic endfeet. BBB’s injuries is a important symbol when central nervous system generate lesion in cerebral ischemical reperfusion injury (CIRI), its’ the injury mechanism including that matrix metalloproteinases’ activity raise induce that basal lamina and extracellular matrix degradation, the augmentation of Aquaporin-4’s expression cause vasogenic edema, destruction of tight junction and decrease of related protein expression lead to raise of BBB permeability, the adhesion molecules and cytokines stimulate the inflammatory reaction, a lot of free radicals production and nitric oxide toxicity can pose the damage of endothelial cells and basal lamina. BBB’s protection mainly by reducing the change of BBB’s morphological structure; decreasing BBB’s permeability; reducing the harmful substances go into BBB; maintaining stabilize of central nervous system’s internal environment. Now the mechanism was constantly expounded that BBB’s injury, adjustment and repair, more and more medicines will be applied to the prevention and treatment of the BBB. In this paper, we will review about research progress on the injury mechanism of BBB and the protective effect of it.

Keywords: Matrix metalloproteinases, Aquaporin, Tight junction, Adhesion molecules, Cytokines.
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International Journal of Biotechnology for Wellness Industries

The Effects and Mechanism of Yinqiao Powder on Upper Respiratory Tract Infection
Pages 57-60
Li-Song Liu, Na Lei, Qing Lin, Wei-Li Wang, Han-Wen Yan and Xiao-Hua Duan

DOI: http://dx.doi.org/10.6000/1927-3037.2015.04.02.2

Published: 28 July 2015

Open Access 


Abstract: Pharmacology of Chinese materia medica use modern scientific methods based on the theory of Traditional Chinese Medicine (TCM) to study the interactional regulations between the body and Chinese materia medica. It aims to explain the mechanism and the scientific basis of the effects of traditional Chinese medicine. Research team has found that Yinqiao Powder has good effect on the mouse model of upper respiratory trace mucosal immunity dysfunction induced by cold stimulation with bacteria and viruses. Therefore, the function of Yinqiao Powder in the prevention and in the treatment of upper respiratory tract infection (AURI) cannot be explained only by antibacterial and antiviral properties. The effects of Yinqiao Powder on AURI may be mediated by the improvement of the function of upper respiratory mucosal immune system.

Keywords: Theory of traditional Chinese medicine, Yinqiao Powder, Exterior syndrome, upper respiratory tract infection, mucosal immune.
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