ijbwi
Abstract : The Edible Mushroom Pleurotus spp.: II. Medicinal Values
Abstract : Antimicrobial, Antibiofilm and Immunomodulatory Activities of Lactobacillus rhamnosus and Lactobacillus gasseri against some Bacterial Pathogens
|
|
Abstract: In this study, two Lactobacillus (LAB) strains namely, Lactobacillus rhamnosus EMCC 1105 (L. rhamnosus) and Lactobacillus gasseri EMCC 1930(L. gasseri) were tested for their antagonistic activities against Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) which are known to be frequently implicated in biofilm infections. The acidic cell free culture supernatant (CFS) of 24 h and 48 h cultures of both LAB stains showed antimicrobial effects against the three pathogens in radial diffusion assay. These effects were abolished upon neutralization of CFS indicating that this effect was due to acids only. Both LAB strains could effectively inhibit the biofilm formation of the three test pathogens and largely replaced them on polystyrene surfaces as demonstrated by crystal violet staining, viable count and scanning electron microscopy. Both of the tested LAB strains could inhibit the protease productivity of S. aureus in 24 h and 48 h dual species-biofilms. The supernatant of 24 h-dual biofilms of P. aeruginosa with L. gasseri also showed a significantly lower protease activity compared to that of P. aeruginosa individual biofilm. Neither LAB strains affected phospholipase C production by the test pathogens when they co-exist during biofilm formation. The different preparations of LAB strains caused no significant change in the levels of gamma interferon expressed by peripheral blood mononuclear cells in response to stimulation by the test pathogens in vitro. In conclusion, L. gasseri and L. rhamnosus can be considered as promising tools for combating biofilm infections. Keywords: Probiotics, Lactobacillus rhamnosus, Lactobacillus gasseri, Biofilm, Antibiofilm, Gamma interferon. |
Abstract : The Role of Food Physics in Fulfilment the Expectations of Up-to-Date Food Technologies and Biotechnologies to Use a Well Balanced Nutrition
|
|
Abstract: The paper deals with some topics of important aspects of food safety and application of principles of food physics in the sector of agriculture, food technology, biotechnology, including questions of well balanced nutrition, as well. One of the most important and widely applied field of biotechnology is the food technology. Food production and processing of quality food and safe food are today of primary importance. Food production is based on the principles of GAP, GMP and GHP. Recently the industrial food processing is focused dominantly on the quality, and one of the basic requirements of the quality is the safety. There are various methods and techniques to produce safe food. The modern food technologies and quality measurements (quality control, quality assurance) involve the application of different physical methods – e.g. high pressure, pulsing electrical field, nondestructive techniques (e.g. NMR, NIR-NIT, PAS, INAA) for chemical composition determination, radiation techniques, nanofiltration and reverse osmosis (RO) – as well. Using e.g. ionizing radiation (nuclear methods) and non-ionizing radiation technologies it is possible to fulfil a lot of important expectations: decrease of microbial contamination, improve of sensory properties, increase of storability, etc. The paper deals with questions of up-to-date, with sport motion combined diet, helping in keeping the health, as well. In the last decades a lot of information were distributed concerning several viewpoints of bodyweight reduction and optimation of bodymaa. How should we know that it is useful and not unhealthy? A suitable Nutrition software (AOPNEI, Analysing and Optimation Program for Nourishment and Energy Intake) – developed at the Department of Food Chemistry and Nutrition of the Faculty of Food Science, Corvinus University – can help. Keywords: Bodymass, food processing, food safety, physical methods, quality assurance, nutrition, radiation methods. |
Abstract : Antimicrobial, Antibiofilm and Immunomodulatory Activities of Lactobacillus rhamnosus and Lactobacillus gasseri against some Bacterial Pathogen
|
|
Abstract: In this study, two Lactobacillus (LAB) strains namely, Lactobacillus rhamnosus EMCC 1105 (L. rhamnosus) and Lactobacillus gasseri EMCC 1930 (L. gasseri) were tested for their antagonistic activities against Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) which are known to be frequently implicated in biofilm infections. The acidic cell free culture supernatant (CFS) of 24 h and 48 h cultures of both LAB stains showed antimicrobial effects against the three pathogens in radial diffusion assay. These effects were abolished upon neutralization of CFS indicating that this effect was due to acids only. Both LAB strains could effectively inhibit the biofilm formation of the three test pathogens and largely replaced them on polystyrene surfaces as demonstrated by crystal violet staining, viable count and scanning electron microscopy. Both of the tested LAB strains could inhibit the protease productivity of S. aureus in 24 h and 48 h dual species-biofilms. The supernatant of 24 h-dual biofilms of P. aeruginosa with L. gasseri also showed a significantly lower protease activity compared to that of P. aeruginosa individual biofilm. Neither LAB strains affected phospholipase C production by the test pathogens when they co-exist during biofilm formation. The different preparations of LAB strains caused no significant change in the levels of gamma interferon expressed by peripheral blood mononuclear cells in response to stimulation by the test pathogens in vitro. In conclusion, L. gasseri and L. rhamnosus can be considered as promising tools for combating biofilm infections. Keywords: Probiotics, Lactobacillus rhamnosus, Lactobacillus gasseri, Biofilm, Antibiofilm, Gamma interferon. |
Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4
